Localization of Platelet-derived Endothelial Cell Growth Factor in Human Placenta and Purification of an Alternatively Processed Form

Overview

Journal Cell Regul

Specialties Biotechnology
Cell Biology
Molecular Biology

Date 1990 Jul 1

PMID 2078568

Citations 7

Authors

K Usuki

L Norberg

E Larsson

K Miyazono

U Hellman

C Wernstedt

K Rubin

C H Heldin

Affiliations

Soon will be listed here.

Abstract

Platelet-derived endothelial cell growth factor (PD-ECGF) was purified to homogeneity from human term placenta, an organ characterized by extensive angiogenesis. N-terminal amino acid sequencing revealed that placental PD-ECGF was proteolytically processed at Thr-6, in contrast to PD-ECGF purified from human platelets, which is processed at Ala-11. The purified factor stimulated porcine aortic endothelial cells as well as two choriocarcinoma cell lines. Immunohistochemical staining revealed that PD-ECGF was present in the connective tissue cells of the placenta. The possibility that PD-ECGF is involved in the development of the placenta is discussed.

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