Insights into Aging Through Measurements of the Drosophila Proteome As a Function of Temperature

Overview

Journal Mech Ageing Dev

Specialty Geriatrics

Date 2010 Aug 25

PMID 20732347

Citations 7

Authors

Rena A S Robinson

John F Kellie

Thomas C Kaufman

David E Clemmer

Affiliations

Soon will be listed here.

Abstract

Drosophila melanogaster is used as a model system to investigate protein changes associated with the aging process under conditions that alter organism lifespan. Changes in the proteome are assessed at various ages in populations of Oregon-R adult males that have mean lifetimes of 47 and 111 days at 28 and 18°C, respectively. Peptide hits detected from strong-cation-exchange and reversed-phase liquid chromatography coupled to tandem mass spectrometry analysis are employed to examine patterns in relative protein expression. Thirty-three proteins were identified as having similar patterns of expression at both temperatures investigated when scaling the organism age to lifespan. In addition, the proteins ferritin 2 light chain homologue and larval serum protein 1β were identified in relatively high abundance and displayed distinctly different patterns of expression between the two temperatures. Overall, the results support the notion that aspects of the aging process may be preprogrammed at the protein level.

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References

Loeb J, NORTHROP J . Is There a Temperature Coefficient for the Duration of Life?. Proc Natl Acad Sci U S A. 1916; 2(8):456-7. PMC: 1091065. DOI: 10.1073/pnas.2.8.456. View

Perkins D, Pappin D, Creasy D, Cottrell J . Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis. 1999; 20(18):3551-67. DOI: 10.1002/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2. View

Zahn J, Sonu R, Vogel H, Crane E, Mazan-Mamczarz K, Rabkin R . Transcriptional profiling of aging in human muscle reveals a common aging signature. PLoS Genet. 2006; 2(7):e115. PMC: 1513263. DOI: 10.1371/journal.pgen.0020115.eor. View

Benes H, Edmondson R, Fink P, Kejzlarova-Lepesant J, Lepesant J, Miles J . Adult expression of the Drosophila Lsp-2 gene. Dev Biol. 1990; 142(1):138-46. DOI: 10.1016/0012-1606(90)90157-e. View

Guarente L, Kenyon C . Genetic pathways that regulate ageing in model organisms. Nature. 2000; 408(6809):255-62. DOI: 10.1038/35041700. View

Rogina B, Benzer S, Helfand S . Drosophila drop-dead mutations accelerate the time course of age-related markers. Proc Natl Acad Sci U S A. 1997; 94(12):6303-6. PMC: 21044. DOI: 10.1073/pnas.94.12.6303. View

Rogina B, Helfand S . Regulation of gene expression is linked to life span in adult Drosophila. Genetics. 1995; 141(3):1043-8. PMC: 1206828. DOI: 10.1093/genetics/141.3.1043. View

Qian W, Jacobs J, Camp 2nd D, Monroe M, Moore R, Gritsenko M . Comparative proteome analyses of human plasma following in vivo lipopolysaccharide administration using multidimensional separations coupled with tandem mass spectrometry. Proteomics. 2005; 5(2):572-84. PMC: 1781926. DOI: 10.1002/pmic.200400942. View

Krishnamurthy J, Torrice C, Ramsey M, Kovalev G, Al-Regaiey K, Su L . Ink4a/Arf expression is a biomarker of aging. J Clin Invest. 2004; 114(9):1299-307. PMC: 524230. DOI: 10.1172/JCI22475. View

10.

Xun Z, Sowell R, Kaufman T, Clemmer D . Protein expression in a Drosophila model of Parkinson's disease. J Proteome Res. 2007; 6(1):348-57. PMC: 2597372. DOI: 10.1021/pr060488o. View

11.

Wheeler J, King V, Tower J . Sequence requirements for upregulated expression of Drosophila hsp70 transgenes during aging. Neurobiol Aging. 2000; 20(5):545-53. DOI: 10.1016/s0197-4580(99)00088-3. View

12.

Sowell R, Hersberger K, Kaufman T, Clemmer D . Examining the proteome of Drosophila across organism lifespan. J Proteome Res. 2007; 6(9):3637-47. DOI: 10.1021/pr070224h. View

13.

McLean J, Krishnakumar S, ODonnell J . Multiple mRNAs from the Punch locus of Drosophila melanogaster encode isoforms of GTP cyclohydrolase I with distinct N-terminal domains. J Biol Chem. 1993; 268(36):27191-7. View

14.

Cohet Y . Epigenetic influences on the lifespan of the Drosophila: existence of an optimal growth temperature for adult longevity. Exp Gerontol. 1975; 10(3-4):181-4. DOI: 10.1016/0531-5565(75)90029-7. View

15.

Pang J, Ginanni N, Dongre A, Hefta S, Opitek G . Biomarker discovery in urine by proteomics. J Proteome Res. 2003; 1(2):161-9. DOI: 10.1021/pr015518w. View

16.

Gao J, Friedrichs M, Dongre A, Opiteck G . Guidelines for the routine application of the peptide hits technique. J Am Soc Mass Spectrom. 2005; 16(8):1231-8. DOI: 10.1016/j.jasms.2004.12.002. View

17.

Ashburner M, Ball C, Blake J, Botstein D, Butler H, Cherry J . Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet. 2000; 25(1):25-9. PMC: 3037419. DOI: 10.1038/75556. View

18.

Miquel J, Lundgren P, Bensch K, ATLAN H . Effects of temperature on the life span, vitality and fine structure of Drosophila melanogaster. Mech Ageing Dev. 1976; 5(5):347-70. DOI: 10.1016/0047-6374(76)90034-8. View

19.

Mackay W, ODonnell J . A genetic analysis of the pteridine biosynthetic enzyme, guanosine triphosphate cyclohydrolase, in Drosophila melanogaster. Genetics. 1983; 105(1):35-53. PMC: 1202150. DOI: 10.1093/genetics/105.1.35. View

20.

Roberts D, Wolfe J, Akam M . The developmental profiles of two major haemolymph proteins from Drosophila melanogaster. J Insect Physiol. 1977; 23(7):871-8. DOI: 10.1016/0022-1910(77)90013-0. View