E. Coli K5 Fermentation and the Preparation of Heparosan, a Bioengineered Heparin Precursor

Overview

Journal Biotechnol Bioeng

Publisher Wiley

Specialty Biochemistry

Date 2010 Aug 19

PMID 20717972

Citations 43

Authors

Zhenyu Wang

Mellisa Ly

Fuming Zhang

Weihong Zhong

Amy Suen

Anne Marie Hickey

Jonathan S Dordick

Robert J Linhardt

Affiliations

Soon will be listed here.

Abstract

Heparosan is an acidic polysaccharide natural product, which serves as the critical precursor in heparin biosynthesis and in the chemoenzymatic synthesis of bioengineered heparin. Heparosan is also the capsular polysaccharide of Escherichia coli K5 strain. The current study was focused on the examination of the fermentation of E. coli K5 with the goal of producing heparosan in high yield and volumetric productivity. The structure and molecular weight properties of this bacterial heparosan were determined using polyacrylamide gel electrophoresis (PAGE) and Fourier transform mass spectrometry. Fermentation of E. coli K5 in a defined medium using exponential fed-batch glucose addition with oxygen enrichment afforded heparosan at 15 g/L having a number average molecular weight of 58,000 Da and a weight average molecular weight of 84,000 Da.

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