Contribution of Coagulases Towards Staphylococcus Aureus Disease and Protective Immunity

Overview

Journal PLoS Pathog

Specialty Microbiology

Date 2010 Aug 12

PMID 20700445

Citations 162

Authors

Alice G Cheng

Molly McAdow

Hwan K Kim

Taeok Bae

Dominique M Missiakas

Olaf Schneewind

Affiliations

Soon will be listed here.

Abstract

The bacterial pathogen Staphylococcus aureus seeds abscesses in host tissues to replicate at the center of these lesions, protected from host immune cells via a pseudocapsule. Using histochemical staining, we identified prothrombin and fibrin within abscesses and pseudocapsules. S. aureus secretes two clotting factors, coagulase (Coa) and von Willebrand factor binding protein (vWbp). We report here that Coa and vWbp together are required for the formation of abscesses. Coa and vWbp promote the non-proteolytic activation of prothrombin and cleavage of fibrinogen, reactions that are inhibited with specific antibody against each of these molecules. Coa and vWbp specific antibodies confer protection against abscess formation and S. aureus lethal bacteremia, suggesting that coagulases function as protective antigens for a staphylococcal vaccine.

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References

Panizzi P, Friedrich R, Fuentes-Prior P, Richter K, Bock P, Bode W . Fibrinogen substrate recognition by staphylocoagulase.(pro)thrombin complexes. J Biol Chem. 2005; 281(2):1179-87. PMC: 2291351. DOI: 10.1074/jbc.M507956200. View

Bjerketorp J, Jacobsson K, Frykberg L . The von Willebrand factor-binding protein (vWbp) of Staphylococcus aureus is a coagulase. FEMS Microbiol Lett. 2004; 234(2):309-14. DOI: 10.1016/j.femsle.2004.03.040. View

Klevens R, Edwards J, Gaynes R . The impact of antimicrobial-resistant, health care-associated infections on mortality in the United States. Clin Infect Dis. 2008; 47(7):927-30. DOI: 10.1086/591698. View

Hussain M, Becker K, von Eiff C, Schrenzel J, Peters G, Herrmann M . Identification and characterization of a novel 38.5-kilodalton cell surface protein of Staphylococcus aureus with extended-spectrum binding activity for extracellular matrix and plasma proteins. J Bacteriol. 2001; 183(23):6778-86. PMC: 95517. DOI: 10.1128/JB.183.23.6778-6786.2001. View

Heilmann C, Herrmann M, Kehrel B, Peters G . Platelet-binding domains in 2 fibrinogen-binding proteins of Staphylococcus aureus identified by phage display. J Infect Dis. 2002; 186(1):32-9. DOI: 10.1086/341081. View

Studier F, Rosenberg A, Dunn J, Dubendorff J . Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 1990; 185:60-89. DOI: 10.1016/0076-6879(90)85008-c. View

Loeb L . The Influence of certain Bacteria on the Coagulation of the Blood. J Med Res. 2009; 10(3):407-19. PMC: 2105975. View

Schneewind O, Model P, Fischetti V . Sorting of protein A to the staphylococcal cell wall. Cell. 1992; 70(2):267-81. DOI: 10.1016/0092-8674(92)90101-h. View

DeBord K, Anderson D, Marketon M, Overheim K, DePaolo R, Ciletti N . Immunogenicity and protective immunity against bubonic plague and pneumonic plague by immunization of mice with the recombinant V10 antigen, a variant of LcrV. Infect Immun. 2006; 74(8):4910-4. PMC: 1539636. DOI: 10.1128/IAI.01860-05. View

10.

Thammavongsa V, Kern J, Missiakas D, Schneewind O . Staphylococcus aureus synthesizes adenosine to escape host immune responses. J Exp Med. 2009; 206(11):2417-27. PMC: 2768845. DOI: 10.1084/jem.20090097. View

11.

Stranger-Jones Y, Bae T, Schneewind O . Vaccine assembly from surface proteins of Staphylococcus aureus. Proc Natl Acad Sci U S A. 2006; 103(45):16942-7. PMC: 1636558. DOI: 10.1073/pnas.0606863103. View

12.

Cheng A, Kim H, Burts M, Krausz T, Schneewind O, Missiakas D . Genetic requirements for Staphylococcus aureus abscess formation and persistence in host tissues. FASEB J. 2009; 23(10):3393-404. PMC: 2747682. DOI: 10.1096/fj.09-135467. View

13.

Harvey R, Degryse E, Stefani L, Schamber F, Cazenave J, Courtney M . Cloning and expression of a cDNA coding for the anticoagulant hirudin from the bloodsucking leech, Hirudo medicinalis. Proc Natl Acad Sci U S A. 1986; 83(4):1084-8. PMC: 323015. DOI: 10.1073/pnas.83.4.1084. View

14.

Mainiero M, Goerke C, Geiger T, Gonser C, Herbert S, Wolz C . Differential target gene activation by the Staphylococcus aureus two-component system saeRS. J Bacteriol. 2009; 192(3):613-23. PMC: 2812464. DOI: 10.1128/JB.01242-09. View

15.

Kinoshita M, Kobayashi N, Nagashima S, Ishino M, Otokozawa S, Mise K . Diversity of staphylocoagulase and identification of novel variants of staphylocoagulase gene in Staphylococcus aureus. Microbiol Immunol. 2008; 52(7):334-48. DOI: 10.1111/j.1348-0421.2008.00045.x. View

16.

Lowy F . Staphylococcus aureus infections. N Engl J Med. 1998; 339(8):520-32. DOI: 10.1056/NEJM199808203390806. View

17.

Geoghegan J, Ganesh V, Smeds E, Liang X, Hook M, Foster T . Molecular characterization of the interaction of staphylococcal microbial surface components recognizing adhesive matrix molecules (MSCRAMM) ClfA and Fbl with fibrinogen. J Biol Chem. 2009; 285(9):6208-16. PMC: 2825416. DOI: 10.1074/jbc.M109.062208. View

18.

Projan S, Nesin M, Dunman P . Staphylococcal vaccines and immunotherapy: to dream the impossible dream?. Curr Opin Pharmacol. 2006; 6(5):473-9. DOI: 10.1016/j.coph.2006.04.005. View

19.

STREITFELD M, SALLMAN B, SHOELSON S . Staphylocoagulase inhibition by pooled human gamma-globulin. Nature. 1959; 184(Suppl 21):1665-6. DOI: 10.1038/1841665a0. View

20.

Kroh H, Panizzi P, Bock P . Von Willebrand factor-binding protein is a hysteretic conformational activator of prothrombin. Proc Natl Acad Sci U S A. 2009; 106(19):7786-91. PMC: 2683071. DOI: 10.1073/pnas.0811750106. View