In Silico Analyses of Proteomic Data Suggest a Role for Heat Shock Proteins in Umbilical Cord Blood Hematopoietic Stem Cells

Overview

Journal Stem Cell Rev Rep

Publisher Springer

Specialty Cell Biology

Date 2010 Aug 11

PMID 20697978

Citations 3

Authors

Angelo DAlessandro

Giuliano Grazzini

Bruno Giardina

Lello Zolla

Affiliations

Soon will be listed here.

Abstract

Umbilical-cord blood (UCB) has growingly become an accepted alternative source of hematopoietic stem cells for transplantation purposes. However, the low cell dose limit within a single unit is still an obstacle hindering the way of a broader diffusion. The real deal is the lack of knowledge about the molecular processes governing the events of expansion and differentiation of these cells. In order to fill this void, several studies were focused on the identification of the peculiar whole protein profile of UCB-derived hematopoietic stem cells. In this review article we provide a referenced list of overall proteins from UCB-derived hematopoietic stem and progenitor cells. This list has been elaborated for pathway and network analyses, along with GO term enrichment for biological and molecular functions, in order to individuate main classes of proteins governing functioning of these cells. From these analyses it seems to emerge a central role for heat shock proteins in immature hematopoietic stem cells. Their role might be relevant in protecting crucial transcription factors which drive proliferation and differentiation towards a specific lineage (e.g. erythroid, myeloid). Hereby we also stress the helpfulness of interactomics elaboration in providing a unified overview of independent proteomics data. It appears that maturation, other than representing a bottleneck to protein expression, could sculpt interaction maps via reducing complexity of immature interactomics profiles.

Citing Articles

Classic and alternative red blood cell storage strategies: seven years of "-omics" investigations.

Zolla L, DAlessandro A, Rinalducci S, Damici G, Pupella S, Vaglio S Blood Transfus. 2014; 13(1):21-31.

PMID: 25369599 PMC: 4317086. DOI: 10.2450/2014.0053-14.

Proteomic Profiling of Ex Vivo Expanded CD34-Positive Haematopoetic Cells Derived from Umbilical Cord Blood.

Falkenberg H, Radke T, Kogler G, Stuhler K Stem Cells Int. 2013; 2013:245695.

PMID: 23606859 PMC: 3622389. DOI: 10.1155/2013/245695.

Role of heat shock proteins in stem cell behavior.

Fan G Prog Mol Biol Transl Sci. 2012; 111:305-22.

PMID: 22917237 PMC: 4422174. DOI: 10.1016/B978-0-12-398459-3.00014-9.

References

Ditton H, Zimmer J, Kamp C, Rajpert-De Meyts E, Vogt P . The AZFa gene DBY (DDX3Y) is widely transcribed but the protein is limited to the male germ cells by translation control. Hum Mol Genet. 2004; 13(19):2333-41. DOI: 10.1093/hmg/ddh240. View

Timperio A, DAlessandro A, Pariset L, Damici G, Valentini A, Zolla L . Comparative proteomics and transcriptomics analyses of livers from two different Bos taurus breeds: "Chianina and Holstein Friesian". J Proteomics. 2009; 73(2):309-22. DOI: 10.1016/j.jprot.2009.09.015. View

Wright C, Chovatiya R, Jameson N, Turner D, Zhu G, Werner S . Pyrimidinone-peptoid hybrid molecules with distinct effects on molecular chaperone function and cell proliferation. Bioorg Med Chem. 2008; 16(6):3291-301. PMC: 2330198. DOI: 10.1016/j.bmc.2007.12.014. View

Olsen J, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P . Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 2006; 127(3):635-48. DOI: 10.1016/j.cell.2006.09.026. View

Rubinstein P, ROSENFIELD R, Adamson J, Stevens C . Stored placental blood for unrelated bone marrow reconstitution. Blood. 1993; 81(7):1679-90. View

Leppa S, Pirkkala L, Saarento H, Sarge K, Sistonen L . Overexpression of HSF2-beta inhibits hemin-induced heat shock gene expression and erythroid differentiation in K562 cells. J Biol Chem. 1997; 272(24):15293-8. DOI: 10.1074/jbc.272.24.15293. View

Garrido C, Fromentin A, Bonnotte B, Favre N, Moutet M, Arrigo A . Heat shock protein 27 enhances the tumorigenicity of immunogenic rat colon carcinoma cell clones. Cancer Res. 1998; 58(23):5495-9. View

Azuma N, Yamaguchi Y, Handa H, Hayakawa M, Kanai A, Yamada M . Missense mutation in the alternative splice region of the PAX6 gene in eye anomalies. Am J Hum Genet. 1999; 65(3):656-63. PMC: 1377971. DOI: 10.1086/302529. View

Calderwood S, Khaleque M, Sawyer D, Ciocca D . Heat shock proteins in cancer: chaperones of tumorigenesis. Trends Biochem Sci. 2006; 31(3):164-72. DOI: 10.1016/j.tibs.2006.01.006. View

10.

Rogers I, Yamanaka N, Casper R . A simplified procedure for hematopoietic stem cell amplification using a serum-free, feeder cell-free culture system. Biol Blood Marrow Transplant. 2008; 14(8):927-37. DOI: 10.1016/j.bbmt.2008.06.002. View

11.

Liu F, Lu J, Fan H, Wang Z, Cui S, Zhang G . Insights into human CD34+ hematopoietic stem/progenitor cells through a systematically proteomic survey coupled with transcriptome. Proteomics. 2006; 6(9):2673-92. DOI: 10.1002/pmic.200500032. View

12.

Zolla L . Proteomics and transfusion medicine. Blood Transfus. 2008; 6(2):67-9. PMC: 2626847. DOI: 10.2450/2008.0003-08. View

13.

Kontush A . Alzheimer's amyloid-beta as a preventive antioxidant for brain lipoproteins. Cell Mol Neurobiol. 2002; 21(4):299-315. PMC: 11533814. DOI: 10.1023/a:1012629603390. View

14.

Armitage J . Bone marrow transplantation. N Engl J Med. 1994; 330(12):827-38. DOI: 10.1056/NEJM199403243301206. View

15.

Al-Shahrour F, Minguez P, Vaquerizas J, Conde L, Dopazo J . BABELOMICS: a suite of web tools for functional annotation and analysis of groups of genes in high-throughput experiments. Nucleic Acids Res. 2005; 33(Web Server issue):W460-4. PMC: 1160217. DOI: 10.1093/nar/gki456. View

16.

Robinson S, Ng J, Niu T, Yang H, McMannis J, Karandish S . Superior ex vivo cord blood expansion following co-culture with bone marrow-derived mesenchymal stem cells. Bone Marrow Transplant. 2006; 37(4):359-66. PMC: 1885539. DOI: 10.1038/sj.bmt.1705258. View

17.

Zenzmaier C, Gesslbauer B, Grobuschek N, Jandrositz A, Preisegger K, Kungl A . Proteomic profiling of human stem cells derived from umbilical cord blood. Biochem Biophys Res Commun. 2005; 328(4):968-72. DOI: 10.1016/j.bbrc.2005.01.044. View

18.

Al-Shahrour F, Minguez P, Tarraga J, Medina I, Alloza E, Montaner D . FatiGO +: a functional profiling tool for genomic data. Integration of functional annotation, regulatory motifs and interaction data with microarray experiments. Nucleic Acids Res. 2007; 35(Web Server issue):W91-6. PMC: 1933151. DOI: 10.1093/nar/gkm260. View

19.

Matsui H, Asou H, Inaba T . Cytokines direct the regulation of Bim mRNA stability by heat-shock cognate protein 70. Mol Cell. 2007; 25(1):99-112. DOI: 10.1016/j.molcel.2006.12.007. View

20.

Sugihara T, Wadhwa R, Kaul S, Mitsui Y . A novel testis-specific metallothionein-like protein, tesmin, is an early marker of male germ cell differentiation. Genomics. 1999; 57(1):130-6. DOI: 10.1006/geno.1999.5756. View