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Dielectrophoretic Separation of Mouse Melanoma Clones

Overview
Journal Biomicrofluidics
Publisher American Institute of Physics
Specialty Biomedical Engineering
Date 2010 Aug 11
PMID 20697600
Citations 16
Authors
Ahmet C Sabuncu
Jie A Liu
Stephen J Beebe
Ali Beskok
Affiliations
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Abstract

Dielectrophoresis (DEP) is employed to differentiate clones of mouse melanoma B16F10 cells. Five clones were tested on microelectrodes. At a specific excitation frequency, clone 1 showed a different DEP response than the other four. Growth rate, melanin content, recovery from cryopreservation, and in vitro invasive studies were performed. Clone 1 is shown to have significantly different melanin content and recovery rate from cryopreservation. This paper reports the ability of DEP to differentiate between two malignant cells of the same origin. Different DEP responses of the two clones could be linked to their melanin content.

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