Two-photon High-resolution Measurement of Partial Pressure of Oxygen in Cerebral Vasculature and Tissue

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2010 Aug 10

PMID 20693997

Citations 186

Authors

Sava Sakadzic

Emmanuel Roussakis

Mohammad A Yaseen

Emiri T Mandeville

Vivek J Srinivasan

Ken Arai

Svetlana Ruvinskaya

Anna Devor

Eng H Lo

Sergei A Vinogradov

David A Boas

Affiliations

Soon will be listed here.

Abstract

Measurements of oxygen partial pressure (pO(2)) with high temporal and spatial resolution in three dimensions is crucial for understanding oxygen delivery and consumption in normal and diseased brain. Among existing pO(2) measurement methods, phosphorescence quenching is optimally suited for the task. However, previous attempts to couple phosphorescence with two-photon laser scanning microscopy have faced substantial difficulties because of extremely low two-photon absorption cross-sections of conventional phosphorescent probes. Here we report to our knowledge the first practical in vivo two-photon high-resolution pO(2) measurements in small rodents' cortical microvasculature and tissue, made possible by combining an optimized imaging system with a two-photon-enhanced phosphorescent nanoprobe. The method features a measurement depth of up to 250 microm, sub-second temporal resolution and requires low probe concentration. The properties of the probe allowed for direct high-resolution measurement of cortical extravascular (tissue) pO(2), opening many possibilities for functional metabolic brain studies.

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