Lotus Leaf Extract and L-carnitine Influence Different Processes During the Adipocyte Life Cycle

Overview

Journal Nutr Metab (Lond)

Publisher Biomed Central

Date 2010 Aug 7

PMID 20687953

Citations 10

Authors

Ralf Siegner

Stefan Heuser

Ursula Holtzmann

Jorn Sohle

Andreas Schepky

Thomas Raschke

Franz Stab

Horst Wenck

Marc Winnefeld

Affiliations

Soon will be listed here.

Abstract

Background: The cellular and molecular mechanisms of adipose tissue biology have been studied extensively over the last two decades. Adipose tissue growth involves both an increase in fat cell size and the formation of mature adipocytes from precursor cells. To investigate how natural substances influence these two processes, we examined the effects of lotus leaf extract (Nelumbo nucifera-extract solution obtained from Silab, France) and L-carnitine on human preadipocytes and adipocytes.

Methods: For our in vitro studies, we used a lotus leaf extract solution alone or in combination with L-carnitine. Utilizing cultured human preadipocytes, we investigated lotus leaf extract solution-induced inhibition of triglyceride incorporation during adipogenesis and possible effects on cell viability. Studies on human adipocytes were performed aiming to elucidate the efficacy of lotus leaf extract solution to stimulate lipolytic activity. To further characterize lotus leaf extract solution-mediated effects, we determined the expression of the transcription factor adipocyte determination and differentiation factor 1 (ADD1/SREBP-1c) on the RNA- and protein level utilizing qRT-PCR and immunofluorescence analysis. Additionally, the effect of L-carnitine on beta-oxidation was analyzed using human preadipocytes and mature adipocytes. Finally, we investigated additive effects of a combination of lotus leaf extract solution and L-carnitine on triglyceride accumulation during preadipocyte/adipocyte differentiation.

Results: Our data showed that incubation of preadipocytes with lotus leaf extract solution significantly decreased triglyceride accumulation during adipogenesis without affecting cell viability. Compared to controls, adipocytes incubated with lotus leaf extract solution exhibited a significant increase in lipolysis-activity. Moreover, cell populations cultivated in the presence of lotus leaf extract solution showed a decrease in adipocyte differentiation capacity as indicated by a decrease in the ADD1/SREBP-1c signal. Importantly, our results demonstrated that a combination of lotus leaf extract solution and L-carnitine reduced triglyceride accumulation to a greater extent compared to incubation with either substance alone.

Conclusions: Overall, our data demonstrate that a combination of lotus leaf extract and L-carnitine reduced triglyceride accumulation in human (pre)adipocytes by affecting different processes during the adipocyte life cycle. For this reason, this combination might represent a treatment option for obesity-related diseases.

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References

Kim Y, Kim K, Kim M, Lee J, Lee K, Park T . A mixture of the aqueous extract of Garcinia cambogia, soy peptide and L: -carnitine reduces the accumulation of visceral fat mass in rats rendered obese by a high fat diet. Genes Nutr. 2008; 2(4):353-8. PMC: 2478482. DOI: 10.1007/s12263-007-0070-1. View

Vaz F, Wanders R . Carnitine biosynthesis in mammals. Biochem J. 2002; 361(Pt 3):417-29. PMC: 1222323. DOI: 10.1042/0264-6021:3610417. View

Rawlings A . Cellulite and its treatment. Int J Cosmet Sci. 2008; 28(3):175-90. DOI: 10.1111/j.1467-2494.2006.00318.x. View

Gregoire F . Adipocyte differentiation: from fibroblast to endocrine cell. Exp Biol Med (Maywood). 2001; 226(11):997-1002. DOI: 10.1177/153537020122601106. View

Rosen E, Walkey C, Puigserver P, Spiegelman B . Transcriptional regulation of adipogenesis. Genes Dev. 2000; 14(11):1293-307. View

Yokoyama C, Wang X, Briggs M, Admon A, Wu J, Hua X . SREBP-1, a basic-helix-loop-helix-leucine zipper protein that controls transcription of the low density lipoprotein receptor gene. Cell. 1993; 75(1):187-97. View

Visscher T, Seidell J . The public health impact of obesity. Annu Rev Public Health. 2001; 22:355-75. DOI: 10.1146/annurev.publhealth.22.1.355. View

Villani R, Gannon J, SELF M, Rich P . L-Carnitine supplementation combined with aerobic training does not promote weight loss in moderately obese women. Int J Sport Nutr Exerc Metab. 2000; 10(2):199-207. DOI: 10.1123/ijsnem.10.2.199. View

Sohle J, Knott A, Holtzmann U, Siegner R, Gronniger E, Schepky A . White Tea extract induces lipolytic activity and inhibits adipogenesis in human subcutaneous (pre)-adipocytes. Nutr Metab (Lond). 2009; 6:20. PMC: 2685800. DOI: 10.1186/1743-7075-6-20. View

10.

Murosaki S, Lee T, Muroyama K, Shin E, Cho S, Yamamoto Y . A combination of caffeine, arginine, soy isoflavones, and L-carnitine enhances both lipolysis and fatty acid oxidation in 3T3-L1 and HepG2 cells in vitro and in KK mice in vivo. J Nutr. 2007; 137(10):2252-7. DOI: 10.1093/jn/137.10.2252. View

11.

Tontonoz P, Kim J, Graves R, Spiegelman B . ADD1: a novel helix-loop-helix transcription factor associated with adipocyte determination and differentiation. Mol Cell Biol. 1993; 13(8):4753-9. PMC: 360101. DOI: 10.1128/mcb.13.8.4753-4759.1993. View

12.

Amin K, Nagy M . Effect of Carnitine and herbal mixture extract on obesity induced by high fat diet in rats. Diabetol Metab Syndr. 2009; 1(1):17. PMC: 2772188. DOI: 10.1186/1758-5996-1-17. View

13.

Ohkoshi E, Miyazaki H, Shindo K, Watanabe H, Yoshida A, Yajima H . Constituents from the leaves of Nelumbo nucifera stimulate lipolysis in the white adipose tissue of mice. Planta Med. 2007; 73(12):1255-9. DOI: 10.1055/s-2007-990223. View

14.

Kashiwada Y, Aoshima A, Ikeshiro Y, Chen Y, Furukawa H, Itoigawa M . Anti-HIV benzylisoquinoline alkaloids and flavonoids from the leaves of Nelumbo nucifera, and structure-activity correlations with related alkaloids. Bioorg Med Chem. 2004; 13(2):443-8. DOI: 10.1016/j.bmc.2004.10.020. View

15.

Langin D . Adipose tissue lipolysis as a metabolic pathway to define pharmacological strategies against obesity and the metabolic syndrome. Pharmacol Res. 2006; 53(6):482-91. DOI: 10.1016/j.phrs.2006.03.009. View

16.

Muller D, Seim H, Kiess W, Loster H, Richter T . Effects of oral L-carnitine supplementation on in vivo long-chain fatty acid oxidation in healthy adults. Metabolism. 2002; 51(11):1389-91. DOI: 10.1053/meta.2002.35181. View

17.

Ono Y, Hattori E, Fukaya Y, Imai S, Ohizumi Y . Anti-obesity effect of Nelumbo nucifera leaves extract in mice and rats. J Ethnopharmacol. 2006; 106(2):238-44. DOI: 10.1016/j.jep.2005.12.036. View

18.

Gronniger E, Wessel S, Kuhn S, Sohle J, Wenck H, Stab F . A new protocol for functional analysis of adipogenesis using reverse transfection technology and time-lapse video microscopy. Cell Biol Int. 2010; 34(7):737-46. DOI: 10.1042/CBI20090299. View

19.

Gregoire F, Smas C, Sul H . Understanding adipocyte differentiation. Physiol Rev. 1998; 78(3):783-809. DOI: 10.1152/physrev.1998.78.3.783. View

20.

Goo H, Choi J, Na D . Simultaneous determination of quercetin and its glycosides from the leaves of Nelumbo nucifera by reversed-phase high-performance liquid chromatography. Arch Pharm Res. 2009; 32(2):201-6. DOI: 10.1007/s12272-009-1136-y. View