Sensitive Enzymatic Quantification of 5-hydroxymethylcytosine in Genomic DNA

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2010 Aug 6

PMID 20685817

Citations 225

Authors

Aleksandra Szwagierczak

Sebastian Bultmann

Christine S Schmidt

Fabio Spada

Heinrich Leonhardt

Affiliations

Soon will be listed here.

Abstract

The recent discovery of genomic 5-hydroxymethylcytosine (hmC) and mutations affecting the respective Tet hydroxylases in leukemia raises fundamental questions about this epigenetic modification. We present a sensitive method for fast quantification of genomic hmC based on specific transfer of radiolabeled glucose to hmC by a purified glucosyltransferase. We determined hmC levels in various adult tissues and differentiating embryonic stem cells and show a correlation with differential expression of tet genes.

Citing Articles

Methods for Detection and Mapping of Methylated and Hydroxymethylated Cytosine in DNA.

Kisil O, Sergeev A, Bacheva A, Zvereva M Biomolecules. 2024; 14(11).

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DNA Methylation Analysis by Bisulfite Pyrosequencing of Mouse Embryonic Fibroblasts with Reprogramming Enhanced by Thyroid Hormones.

Santamarina-Ojeda P, Fernandez A, Fraga M, Perez R Methods Mol Biol. 2024; 2876:131-147.

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Robust Bisulfite-Free Single-Molecule Real-Time Sequencing of Methyldeoxycytidine Based on a Novel hpTet3 Enzyme.

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5-Hydroxymethylcytosine: Far Beyond the Intermediate of DNA Demethylation.

Zheng K, Lyu Z, Chen J, Chen G Int J Mol Sci. 2024; 25(21).

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Loss of TET Activity in the Postnatal Mouse Brain Perturbs Synaptic Gene Expression and Impairs Cognitive Function.

Liu J, Zhang Z, Zhu Z, Li K, Xu Q, Zhang J Neurosci Bull. 2024; 40(11):1699-1712.

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