Antiosteoporotic Effects and Proteomic Characterization of the Target and Mechanism of an Er-Xian Decoction on Osteoblastic UMR-106 and Osteoclasts Induced from RAW264.7

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2010 Jul 27

PMID 20657386

Citations 8

Authors

Zheng Zhu

Li-Ming Xue

Ting Han

Lei Jiao

Lu-Ping Qin

Yu-Shan Li

Han-Chen Zheng

Qiao-Yan Zhang

Affiliations

Soon will be listed here.

Abstract

Er-Xian Decoction (EXD) has been used for the treatment of osteoporosis disorders, menopausal syndrome, and other aging diseases in the Chinese traditional healthcare system. However, the targets and mechanism of action have not been clarified. This study was designed to investigate the effects and possible target proteins of EXD on osteoblastic UMR-106 cells and osteoclasts induced from RAW264.7 cells using the proteomic analysis technique. We found that EXD at a concentration of 50-200 microg/mL significantly enhanced osteoblastic UMR-106 cell proliferation, alkaline phosphatase (ALP) activity and formation of bone nodules, and decreased tartrate-resistant acid phosphatase (TRAP) activity and the bone resorption action of osteoclasts induced from RAW 264.7 cells. In EXD-treated osteoblasts, there were increases in the expression of heat-shock protein 1, high mobility group protein (Hmgb1), acidic ribosomal phosphoprotein P0, histone 2, carbonyl reductase 1, ATP synthase, aldolase A, and Rho GDP dissociation inhibitor (GDI)-alpha; and reduction in the expression of carbonic anhydrase 3, prohibitin, hemiferrin, far upstream element (FUSE)-binding protein. In EXD-treated osteoclasts, there were increases in the expression of vimentin, protein disulfide isomerase associated 3 and alpha-fetoprotein; and reduction in the expression of calnexin. These results indicated that EXD modulates bone metabolism through regulation of osteoblastic proliferation, apoptosis, and cell activation, and osteoclastic protein folding and aggregation.

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References

Nian H, Qin L, Zhang Q, Zheng H, Yu Y, Huang B . Antiosteoporotic activity of Er-Xian Decoction, a traditional Chinese herbal formula, in ovariectomized rats. J Ethnopharmacol. 2006; 108(1):96-102. DOI: 10.1016/j.jep.2006.04.020. View

Delom F, Fessart D, Chevet E . Regulation of calnexin sub-cellular localization modulates endoplasmic reticulum stress-induced apoptosis in MCF-7 cells. Apoptosis. 2007; 12(2):293-305. DOI: 10.1007/s10495-006-0625-4. View

Owen T, Aronow M, Shalhoub V, Barone L, Wilming L, Tassinari M . Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol. 1990; 143(3):420-30. DOI: 10.1002/jcp.1041430304. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Binkert C, Demetriou M, Sukhu B, Szweras M, Tenenbaum H, Dennis J . Regulation of osteogenesis by fetuin. J Biol Chem. 1999; 274(40):28514-20. DOI: 10.1074/jbc.274.40.28514. View

Ishizaki H, Togawa A, Tanaka-Okamoto M, Hori K, Nishimura M, Hamaguchi A . Defective chemokine-directed lymphocyte migration and development in the absence of Rho guanosine diphosphate-dissociation inhibitors alpha and beta. J Immunol. 2006; 177(12):8512-21. DOI: 10.4049/jimmunol.177.12.8512. View

Liu Q, Shi J, Yang Y, Fang Z, Liang S, Guo R . [Effects on secretory function of rat gonad by Erxian decoction and its disassembled prescriptions]. Zhongguo Zhong Yao Za Zhi. 2005; 30(13):1023-6. View

Merkwirth C, Langer T . Prohibitin function within mitochondria: essential roles for cell proliferation and cristae morphogenesis. Biochim Biophys Acta. 2008; 1793(1):27-32. DOI: 10.1016/j.bbamcr.2008.05.013. View

Qin L, Han T, Zhang Q, Cao D, Nian H, Rahman K . Antiosteoporotic chemical constituents from Er-Xian Decoction, a traditional Chinese herbal formula. J Ethnopharmacol. 2008; 118(2):271-9. DOI: 10.1016/j.jep.2008.04.009. View

10.

Zambonin Zallone A, Teti A, Primavera M . Isolated osteoclasts in primary culture: first observations on structure and survival in culture media. Anat Embryol (Berl). 1982; 165(3):405-13. DOI: 10.1007/BF00305576. View

11.

Forrest G, Gonzalez B . Carbonyl reductase. Chem Biol Interact. 2001; 129(1-2):21-40. DOI: 10.1016/s0009-2797(00)00196-4. View

12.

Collin-Osdoby P, Yu X, Zheng H, Osdoby P . RANKL-mediated osteoclast formation from murine RAW 264.7 cells. Methods Mol Med. 2003; 80:153-66. DOI: 10.1385/1-59259-366-6:153. View

13.

Zheng X, Hong L, Shi L, Guo J, Sun Z, Zhou J . Proteomics analysis of host cells infected with infectious bursal disease virus. Mol Cell Proteomics. 2007; 7(3):612-25. DOI: 10.1074/mcp.M700396-MCP200. View

14.

Pfeilschifter J, Chenu C, Bird A, Mundy G, Roodman G . Interleukin-1 and tumor necrosis factor stimulate the formation of human osteoclastlike cells in vitro. J Bone Miner Res. 1989; 4(1):113-8. DOI: 10.1002/jbmr.5650040116. View

15.

Pedersen P . Transport ATPases: structure, motors, mechanism and medicine: a brief overview. J Bioenerg Biomembr. 2006; 37(6):349-57. DOI: 10.1007/s10863-005-9470-3. View

16.

Weilbaecher K, Motyckova G, Huber W, Takemoto C, Hemesath T, Xu Y . Linkage of M-CSF signaling to Mitf, TFE3, and the osteoclast defect in Mitf(mi/mi) mice. Mol Cell. 2001; 8(4):749-58. DOI: 10.1016/s1097-2765(01)00360-4. View

17.

Vignesh R, Djody S, Jayasudha E, Gopalakrishnan V, Ilangovan R, Balaganesh M . Effect of ethanol on human osteosarcoma cell proliferation, differentiation and mineralization. Toxicology. 2006; 220(1):63-70. DOI: 10.1016/j.tox.2005.11.026. View

18.

Lindquist S, Craig E . The heat-shock proteins. Annu Rev Genet. 1988; 22:631-77. DOI: 10.1146/annurev.ge.22.120188.003215. View

19.

Lehenkari P, Hentunen T, Laitala-Leinonen T, Tuukkanen J, Vaananen H . Carbonic anhydrase II plays a major role in osteoclast differentiation and bone resorption by effecting the steady state intracellular pH and Ca2+. Exp Cell Res. 1998; 242(1):128-37. DOI: 10.1006/excr.1998.4071. View

20.

Kim S, Jun S, Jang H, Lim S . Identification of parathyroid hormone-regulated proteins in mouse bone marrow cells by proteomics. Biochem Biophys Res Commun. 2005; 330(2):423-9. DOI: 10.1016/j.bbrc.2005.02.173. View