The 7a Accessory Protein of Severe Acute Respiratory Syndrome Coronavirus Acts As an RNA Silencing Suppressor

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2010 Jul 16

PMID 20631126

Citations 29

Authors

Sumona Karjee

Ankita Minhas

Vikas Sood

Sanket S Ponia

Akhil C Banerjea

Vincent T K Chow

Sunil K Mukherjee

Sunil K Lal

Affiliations

Soon will be listed here.

Abstract

RNA silencing suppressors (RSSs) are well studied for plant viruses but are not well defined to date for animal viruses. Here, we have identified an RSS from a medically important positive-sense mammalian virus, Severe acute respiratory syndrome coronavirus. The viral 7a accessory protein suppressed both transgene and virus-induced gene silencing by reducing the levels of small interfering RNA (siRNA). The suppression of silencing was analyzed by two independent assays, and the middle region (amino acids [aa] 32 to 89) of 7a was responsible for suppression. Finally, the RNA suppression property and the enhancement of heterologous replicon activity by the 7a protein were confirmed for animal cell lines.

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