The Reaction of Lupinus Angustifolius L. Root Meristematic Cell Nucleoli to Lead

Overview

Journal Protoplasma

Publisher Springer

Specialty Biology

Date 2010 Jul 14

PMID 20625779

Citations 4

Authors

Lucja Balcerzak

Slawa Glinska

Miroslaw Godlewski

Affiliations

Soon will be listed here.

Abstract

The effect of 2-48 h treatment of Lupinus angustifolius L. roots with lead nitrate at the concentration of 10(-4) M on the nucleoli in meristematic cells was investigated. In the lead presence the number of ring-shaped as well as segregated nucleoli increased especially after 12-48 h of treatment, while spindle-shaped nucleoli appeared after 24 h and 48 h. Lead presence also increased the frequency of cells with silver-stained particles in the nucleus and the number of these particles especially from the 12th hour of treatment. It was accompanied by significant decline of nucleolar area. Analysis of these cells in transmission electron microscope confirmed the presence of ring-shaped and segregated nucleoli. Moreover, electron microscopy revealed compact structure nucleoli without granular component. Additionally, one to three oval-shaped fibrillar structures attached to nucleolus or lying free in the nucleoplasm were visible. The possible mechanism of lead toxicity to the nucleolus is briefly discussed.

Citing Articles

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References

Marcano L, Carruyo I, Del Campo A, Montiel X . Effect of cadmium on the nucleoli of meristematic cells of onion Allium cepa L: an ultrastructural study. Environ Res. 2002; 88(1):30-5. DOI: 10.1006/enrs.2001.4316. View

Jiang W, Liu D, Li H . Effects of Cu2+ on root growth, cell division, and nucleolus of Helianthus annuus L. Sci Total Environ. 2000; 256(1):59-65. DOI: 10.1016/s0048-9697(00)00470-8. View

Kuran H, Kononowicz A . Effect of starvation on the genetic activity of nucleus and nucleolar organizer. Folia Histochem Cytochem (Krakow). 1979; 17(1):59-68. View

Risueno M, Medina F . The nucleolar structure in plant cells. Revis Biol Celular. 1986; 7:1-154. View

Wang F, Shang G, Hao S, Jiao M . Dynamic change and derivation process of FC and DFC through G1, S and G2 phases in HeLa cells. Cell Biol Int. 2006; 30(10):836-40. DOI: 10.1016/j.cellbi.2006.05.014. View

Glater R, Hernandez Jr L . Lead detection in living plant tissue using a new histochemical method. J Air Pollut Control Assoc. 1972; 22(6):463-7. DOI: 10.1080/00022470.1972.10469663. View

Liu D, Jiang W, Wang W, Zhao F, Lu C . Effects of lead on root growth, cell division, and nucleolus of Allium cepa. Environ Pollut. 1994; 86(1):1-4. DOI: 10.1016/0269-7491(94)90002-7. View

Shang G, Wang F, Hao S, Jiao M . Dynamic changes of nucleolar DNA configuration and distribution during the cell cycle in Allium sativum cells. Micron. 2009; 40(4):449-54. DOI: 10.1016/j.micron.2009.01.001. View

Collins A . The comet assay for DNA damage and repair: principles, applications, and limitations. Mol Biotechnol. 2004; 26(3):249-61. DOI: 10.1385/MB:26:3:249. View

10.

Howell W, Black D . Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia. 1980; 36(8):1014-5. DOI: 10.1007/BF01953855. View

11.

Tice R, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H . Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen. 2000; 35(3):206-21. DOI: 10.1002/(sici)1098-2280(2000)35:3<206::aid-em8>3.0.co;2-j. View

12.

Reynolds E . The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963; 17:208-12. PMC: 2106263. DOI: 10.1083/jcb.17.1.208. View

13.

Carruyo I, Fernandez Y, Marcano L, Montiel X, Torrealba Z . Correlation of toxicity with lead content in root tip cells (Allium cepa L.). Biol Trace Elem Res. 2008; 125(3):276-85. DOI: 10.1007/s12011-008-8175-y. View

14.

Sengar R, Gautam M, Sengar R, Garg S, Sengar K, Chaudhary R . Lead stress effects on physiobiochemical activities of higher plants. Rev Environ Contam Toxicol. 2008; 196:73-93. DOI: 10.1007/978-0-387-78444-1_3. View

15.

Glinska S, Bartczak M, Oleksiak S, Wolska A, Gabara B, Posmyk M . Effects of anthocyanin-rich extract from red cabbage leaves on meristematic cells of Allium cepa L. roots treated with heavy metals. Ecotoxicol Environ Saf. 2007; 68(3):343-50. DOI: 10.1016/j.ecoenv.2007.02.004. View

16.

Jiang W, Liu D . Effects of Pb(2+) on root growth, cell division, and nucleolus of Zea mays L. Bull Environ Contam Toxicol. 2000; 65(6):786-93. DOI: 10.1007/s0012800191. View

17.

Stepinski D . Organization of the nucleoli of soybean root meristematic cells at different states of their activity. Micron. 2010; 41(4):283-8. DOI: 10.1016/j.micron.2009.11.011. View

18.

Derenzini M, Farabegoli F, Trere D . Localization of DNA in the fibrillar components of the nucleolus: a cytochemical and morphometric study. J Histochem Cytochem. 1993; 41(6):829-36. DOI: 10.1177/41.6.8315275. View

19.

Thiry M, Lafontaine D . Birth of a nucleolus: the evolution of nucleolar compartments. Trends Cell Biol. 2005; 15(4):194-9. DOI: 10.1016/j.tcb.2005.02.007. View