Long Noncoding RNA As Modular Scaffold of Histone Modification Complexes

Overview

Journal Science

Specialty Science

Date 2010 Jul 10

PMID 20616235

Citations 1829

Authors

Miao-Chih Tsai

Ohad Manor

Yue Wan

Nima Mosammaparast

Jordon K Wang

Fei Lan

Yang Shi

Eran Segal

Howard Y Chang

Affiliations

Soon will be listed here.

Abstract

Long intergenic noncoding RNAs (lincRNAs) regulate chromatin states and epigenetic inheritance. Here, we show that the lincRNA HOTAIR serves as a scaffold for at least two distinct histone modification complexes. A 5' domain of HOTAIR binds polycomb repressive complex 2 (PRC2), whereas a 3' domain of HOTAIR binds the LSD1/CoREST/REST complex. The ability to tether two distinct complexes enables RNA-mediated assembly of PRC2 and LSD1 and coordinates targeting of PRC2 and LSD1 to chromatin for coupled histone H3 lysine 27 methylation and lysine 4 demethylation. Our results suggest that lincRNAs may serve as scaffolds by providing binding surfaces to assemble select histone modification enzymes, thereby specifying the pattern of histone modifications on target genes.

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