Ultrahigh Sensitive Optical Microangiography for in Vivo Imaging of Microcirculations Within Human Skin Tissue Beds

Overview

Journal Opt Express

Specialties Biophysics
Ophthalmology

Date 2010 Jul 1

PMID 20588668

Citations 158

Authors

Lin An

Jia Qin

Ruikang K Wang

Affiliations

Soon will be listed here.

Abstract

In this paper, we demonstrate for the first time that the detailed cutaneous blood flow at capillary level within dermis of human skin can be imaged by optical micro-angiography (OMAG) technique. A novel scanning protocol, i.e. fast B scan mode is used to achieve the capillary flow imaging. We employ a 1310nm system to scan the skin tissue at an imaging rate of 300 frames per second, which requires only ~5 sec to complete one 3D imaging of capillary blood flow within skin. The technique is sensitive enough to image the very slow blood flows at ~4 microm/sec. The promising results show a great potential of OMAG's role in the diagnosis, treatment and management of human skin diseases.

Citing Articles

Dynamic optical coherence tomography for imaging acute wound healing.

Schuh S, Berger M, Schiele S, Rubeck A, Muller G, Gonzalez J Int Wound J. 2024; 21(8):e70015.

PMID: 39165043 PMC: 11336043. DOI: 10.1111/iwj.70015.

Optical coherence tomography.

Bouma B, de Boer J, Huang D, Jang I, Yonetsu T, Leggett C Nat Rev Methods Primers. 2023; 2.

PMID: 36751306 PMC: 9901537. DOI: 10.1038/s43586-022-00162-2.

Integrating a pressure sensor with an OCT handheld probe to facilitate imaging of microvascular information in skin tissue beds.

Shi Y, Lu J, Le N, Wang R Biomed Opt Express. 2023; 13(11):6153-6166.

PMID: 36733756 PMC: 9872897. DOI: 10.1364/BOE.473013.

High resolution imaging and quantification of the nailfold microvasculature using optical coherence tomography angiography (OCTA) and capillaroscopy: a preliminary study in healthy subjects.

Dong L, Wei Y, Lan G, Chen J, Xu J, Qin J Quant Imaging Med Surg. 2022; 12(3):1844-1858.

PMID: 35284284 PMC: 8899956. DOI: 10.21037/qims-21-672.

Blood vessel tail artifacts suppression in optical coherence tomography angiography.

Li Y, Tang J Neurophotonics. 2022; 9(2):021906.

PMID: 35106321 PMC: 8785979. DOI: 10.1117/1.NPh.9.2.021906.

References

Zhang H, Maslov K, Stoica G, Wang L . Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging. Nat Biotechnol. 2006; 24(7):848-51. DOI: 10.1038/nbt1220. View

Braverman I . The cutaneous microcirculation. J Investig Dermatol Symp Proc. 2001; 5(1):3-9. DOI: 10.1046/j.1087-0024.2000.00010.x. View

Mariampillai A, Standish B, Moriyama E, Khurana M, Munce N, Leung M . Speckle variance detection of microvasculature using swept-source optical coherence tomography. Opt Lett. 2008; 33(13):1530-2. DOI: 10.1364/ol.33.001530. View

Fingler J, Schwartz D, Yang C, Fraser S . Mobility and transverse flow visualization using phase variance contrast with spectral domain optical coherence tomography. Opt Express. 2009; 15(20):12636-53. DOI: 10.1364/oe.15.012636. View

Grulkowski I, Gorczynska I, Szkulmowski M, Szlag D, Szkulmowska A, Leitgeb R . Scanning protocols dedicated to smart velocity ranging in spectral OCT. Opt Express. 2010; 17(26):23736-54. DOI: 10.1364/OE.17.023736. View

Zhao Y, Chen Z, Ding Z, Ren H, Nelson J . Real-time phase-resolved functional optical coherence tomography by use of optical Hilbert transformation. Opt Lett. 2007; 27(2):98-100. DOI: 10.1364/ol.27.000098. View

Ren H, Sun T, MacDonald D, Cobb M, Li X . Real-time in vivo blood-flow imaging by moving-scatterer-sensitive spectral-domain optical Doppler tomography. Opt Lett. 2006; 31(7):927-9. DOI: 10.1364/ol.31.000927. View

Ren H, Li X . Clutter rejection filters for optical Doppler tomography. Opt Express. 2009; 14(13):6103-12. DOI: 10.1364/oe.14.006103. View

Bachmann A, Villiger M, Blatter C, Lasser T, Leitgeb R . Resonant Doppler flow imaging and optical vivisection of retinal blood vessels. Opt Express. 2009; 15(2):408-22. DOI: 10.1364/oe.15.000408. View

10.

Wang R . Fourier domain optical coherence tomography achieves full range complex imaging in vivo by introducing a carrier frequency during scanning. Phys Med Biol. 2007; 52(19):5897-907. DOI: 10.1088/0031-9155/52/19/011. View

11.

Tao Y, Kennedy K, Izatt J . Velocity-resolved 3D retinal microvessel imaging using single-pass flow imaging spectral domain optical coherence tomography. Opt Express. 2009; 17(5):4177-88. PMC: 2723161. DOI: 10.1364/oe.17.004177. View

12.

Wang R, An L . Doppler optical micro-angiography for volumetric imaging of vascular perfusion in vivo. Opt Express. 2009; 17(11):8926-40. PMC: 2714191. DOI: 10.1364/oe.17.008926. View

13.

Vakoc B, Lanning R, Tyrrell J, Padera T, Bartlett L, Stylianopoulos T . Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging. Nat Med. 2009; 15(10):1219-23. PMC: 2759417. DOI: 10.1038/nm.1971. View

14.

Szkulmowski M, Szkulmowska A, Bajraszewski T, Kowalczyk A, Wojtkowski M . Flow velocity estimation using joint Spectral and Time domain Optical Coherence Tomography. Opt Express. 2008; 16(9):6008-25. DOI: 10.1364/oe.16.006008. View

15.

An L, Wang R . In vivo volumetric imaging of vascular perfusion within human retina and choroids with optical micro-angiography. Opt Express. 2008; 16(15):11438-52. DOI: 10.1364/oe.16.011438. View

16.

Szkulmowska A, Szkulmowski M, Szlag D, Kowalczyk A, Wojtkowski M . Three-dimensional quantitative imaging of retinal and choroidal blood flow velocity using joint Spectral and Time domain Optical Coherence Tomography. Opt Express. 2009; 17(13):10584-98. DOI: 10.1364/oe.17.010584. View

17.

Vakoc B, Yun S, de Boer J, Tearney G, Bouma B . Phase-resolved optical frequency domain imaging. Opt Express. 2009; 13(14):5483-93. PMC: 2705336. DOI: 10.1364/opex.13.005483. View

18.

Tao Y, Davis A, Izatt J . Single-pass volumetric bidirectional blood flow imaging spectral domain optical coherence tomography using a modified Hilbert transform. Opt Express. 2008; 16(16):12350-61. DOI: 10.1364/oe.16.012350. View

19.

Wang R, Hurst S . Mapping of cerebro-vascular blood perfusion in mice with skin and skull intact by Optical Micro-AngioGraphy at 1.3 mum wavelength. Opt Express. 2009; 15(18):11402-12. DOI: 10.1364/oe.15.011402. View

20.

Wang R, Jacques S, Ma Z, Hurst S, Hanson S, Gruber A . Three dimensional optical angiography. Opt Express. 2009; 15(7):4083-97. DOI: 10.1364/oe.15.004083. View