Cytotoxic Effects of Resin-modified Orthodontic Band Adhesives. Are They Safe?

Overview

Journal Angle Orthod

Specialty Dentistry

Date 2010 Jun 29

PMID 20578860

Citations 2

Authors

Siddik Malkoc

Bayram Corekci

Hayriye Esra Botsali

Muhammet Yalcin

Abdulkadir Sengun

Affiliations

Soon will be listed here.

Abstract

Objective: To evaluate the cytotoxic effects of three different resin-modified orthodontic band adhesives.

Materials And Methods: Three resin-modified orthodontic band adhesives (Bisco Ortho Band Paste LC, Multi-Cure Glass Ionomer Band Cement, and Transbond Plus Light Cure Band Adhesive) were prepared and the samples were extracted in 3 mL of Basal Medium Eagle with 10% newborn calf serum for 24 hours. The L929 cells were plated (25,000 cells/mL) in wells of 96-well dishes and maintained in a humidified incubator for 24 hours at 37 degrees C, 5% CO(2), and 95% air. After 24-hour incubation of the cells, the incubation medium was replaced by the immersed medium in which the samples were stored. Then L929 cells were incubated in contact with eluates for 24 hours. The cell mitochondrial activity was evaluated by the methyltetrazolium test. Twelve wells were used for each specimen, and methyltetrazolium tests were applied two times. The data were statistically analyzed using one-way analysis of variance and Tukey Honestly Significantly Different tests.

Results: Results with L929 fibroblasts demonstrated that all freshly prepared resin-modified orthodontic band adhesive materials reduced vital cell numbers (P > .05), in comparison to the control group. Our data demonstrate that all materials showed significant cytotoxicity compared to the control group.

Conclusions: The results indicate that all materials showed significant cytotoxicity compared to the control group, and further studies using different test methods are needed for all resin-modified orthodontic band adhesives.

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