Cytotoxicity of Dental Adhesives in Vitro

Overview

Journal Eur J Dent

Publisher Thieme

Specialty Dentistry

Date 2009 Mar 6

PMID 19262725

Citations 18

Authors

Elisabeth A Koulaouzidou

Maria Helvatjoglu-Antoniades

George Palaghias

Artemis Karanika-Kouma

Dimitrios Antoniades

Affiliations

Soon will be listed here.

Abstract

Objectives: The purpose of this study was to evaluate the cytotoxic effect of six dental adhesives (Admira Bond, Clearfil Liner Bond 2V, ED Primer II, Fuji Bond LC, Gluma Comfort Bond, and NanoBond) applied to cell cultures.

Methods: The experiments were performed on two cell lines, rat pulp cells (RPC-C2A) and human lung fibroblasts (MRC5). Samples of the adhesives were light-cured and placed in culture medium for 24 hours. The extraction media was applied on the RPC-C2A and the MRC5 cells. Complete medium was used as a control. Cytotoxicity was evaluated with a modified sulforhodamine B (SRB) assay after 24 hours of exposure.

Results: The cell survival of RPC-C2A cells exposed to Fuji Bond LC, NanoBond, Clearfil Liner Bond 2V, ED Primer II, Admira Bond and Gluma Comfort Bond was 73%, 67%, 50%, 20%, 18% and 5% respectively, relative to the cell survival with the control medium. In the MRC5 cell line, the relative survival was 98%, 80%, 72%, 41%, 19% and 7% after exposure to NanoBond, Fuji Bond LC, Clearfil Liner Bond 2V, ED Primer II, Admira Bond and Gluma Comfort Bond, respectively.

Conclusions: Different types of dental adhesives showed different cytotoxic effects on cells in vitro. The self-etch adhesives were superior in terms of cytotoxicity. The different cytotoxic effects of dental adhesives should be considered when selecting an appropriate adhesive for operative restorations.

Citing Articles

Effects of Adper Scotchbond 1 XT, Clearfil SE Bond 2 and Scotchbond Universal in Odontoblasts.

Cardoso M, Coelho A, Marto C, Goncalves A, Paula A, Ribeiro A Materials (Basel). 2021; 14(21).

PMID: 34771964 PMC: 8585417. DOI: 10.3390/ma14216435.

Comparative study of different cytotoxicity of bonding systems with different dentin thickness on L929 cell line: An experimental study.

Nasseri E, Eskandarizadeh A Dent Res J (Isfahan). 2021; 17(6):424-432.

PMID: 33889347 PMC: 8045521.

The Cytotoxicity and Genotoxicity of Three Dental Universal Adhesives-An In Vitro Study.

Wawrzynkiewicz A, Rozpedek-Kaminska W, Galita G, Lukomska-Szymanska M, Lapinska B, Sokolowski J Int J Mol Sci. 2020; 21(11).

PMID: 32486393 PMC: 7312029. DOI: 10.3390/ijms21113950.

Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study.

Frob L, Ruttermann S, Romanos G, Herrmann E, Gerhardt-Szep S Materials (Basel). 2020; 13(2).

PMID: 31963535 PMC: 7013582. DOI: 10.3390/ma13020452.

Cytotoxicity of Etch-and-Rinse, Self-Etch, and Universal Dental Adhesive Systems in Fibroblast Cell Line 3T3.

Pupo Y, Bernardo C, de Souza F, MichEl M, Ribeiro C, Germano S Scanning. 2017; 2017:9650420.

PMID: 29109829 PMC: 5662068. DOI: 10.1155/2017/9650420.

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