Concentration of Acrylamide in a Polyacrylamide Gel Affects VP4 Gene Coding Assignment of Group A Equine Rotavirus Strains with P[12] Specificity

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2010 Jun 25

PMID 20573245

Authors

LaShanda M Long-Croal

Xiaobo Wen

Eileen N Ostlund

Yasutaka Hoshino

Affiliations

Soon will be listed here.

Abstract

Background: It is universally acknowledged that genome segment 4 of group A rotavirus, the major etiologic agent of severe diarrhea in infants and neonatal farm animals, encodes outer capsid neutralization and protective antigen VP4.

Results: To determine which genome segment of three group A equine rotavirus strains (H-2, FI-14 and FI-23) with P[12] specificity encodes the VP4, we analyzed dsRNAs of strains H-2, FI-14 and FI-23 as well as their reassortants by polyacrylamide gel electrophoresis (PAGE) at varying concentrations of acrylamide. The relative position of the VP4 gene of the three equine P[12] strains varied (either genome segment 3 or 4) depending upon the concentration of acrylamide. The VP4 gene bearing P[3], P[4], P[6], P[7], P[8] or P[18] specificity did not exhibit this phenomenon when the PAGE running conditions were varied.

Conclusions: The concentration of acrylamide in a PAGE gel affected VP4 gene coding assignment of equine rotavirus strains bearing P[12] specificity.

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