A Non-sulfated Chondroitin Stabilizes Membrane Tubulation in Cnidarian Organelles

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2010 Jun 12

PMID 20538610

Citations 19

Authors

Patrizia Adamczyk

Claudia Zenkert

Prakash G Balasubramanian

Shuhei Yamada

Saori Murakoshi

Kazuyuki Sugahara

Jung Shan Hwang

Takashi Gojobori

Thomas W Holstein

Suat Ozbek

Affiliations

Soon will be listed here.

Abstract

Membrane tubulation is generally associated with rearrangements of the cytoskeleton and other cytoplasmic factors. Little is known about the contribution of extracellular matrix components to this process. Here, we demonstrate an essential role of proteoglycans in the tubulation of the cnidarian nematocyst vesicle. The morphogenesis of this extrusive organelle takes place inside a giant post-Golgi vesicle, which topologically represents extracellular space. This process includes the formation of a complex collagenous capsule structure that elongates into a long tubule, which invaginates after its completion. We show that a non-sulfated chondroitin appears as a scaffold in early morphogenesis of all nematocyst types in Hydra and Nematostella. It accompanies the tubulation of the vesicle membrane forming a provisional tubule structure, which after invagination matures by collagen incorporation. Inhibition of chondroitin synthesis by beta-xylosides arrests nematocyst morphogenesis at different stages of tubule outgrowth resulting in retention of tubule material and a depletion of mature capsules in the tentacles of hydra. Our data suggest a conserved role of proteoglycans in the stabilization of a membrane protrusion as an essential step in organelle morphogenesis.

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