Isolation and Characterization of Klebsiella Pneumoniae Specific Bacteriophages from Sewage Samples

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 2010 Jun 8

PMID 20526833

Citations 19

Authors

S Kumari

K Harjai

S Chhibber

Affiliations

Soon will be listed here.

Abstract

Five bacteriophages (Kpn5, Kpn12, Kpn13, Kpn17 and Kpn22), each having specificity against Klebsiella pneumoniae strain B5055, were isolated from sewage samples and characterized in terms of growth characteristics, genetic material, morphology and structural proteins. Adsorption rate as well as single step growth curve experiments showed variation among phages. Restriction enzyme digestion of DNA confirmed the presence of double stranded DNA as well as the heterogeneous nature of genetic material. RAPD-PCR was performed to further distinguish these closely related phages. Their genome fingerprint confirmed their diversity. Transmission electron microscopy, on the other hand, showed their morphological similarity; they were assigned to family Podoviridae, order Caudovirales on the basis of their head and tail morphology. Structural proteins resolved on SDS-PAGE showed the presence of similar major outer membrane proteins. The bacteriophages, belonging to Podoviridae family with short stumpy tails, were found to be nontoxic to mice. They showed maximum count in various organs at 6 h post inoculation, which persisted till 36 h. These phages thus have the potential to be used for phage therapy.

Citing Articles

DeepPBI-KG: a deep learning method for the prediction of phage-bacteria interactions based on key genes.

Wei T, Lu C, Du H, Yang Q, Qi X, Liu Y Brief Bioinform. 2024; 25(6).

PMID: 39344712 PMC: 11440089. DOI: 10.1093/bib/bbae484.

Characterization of ES10 lytic bacteriophage isolated from hospital waste against multidrug-resistant uropathogenic .

Nawaz A, Zafar S, Alessa A, Khalid N, Shahzadi M, Majid A Front Microbiol. 2024; 15:1320974.

PMID: 38525078 PMC: 10957765. DOI: 10.3389/fmicb.2024.1320974.

Isolation of virulent phages against multidrug-resistant Acinetobacter baumannii recovered from inanimate objects of Jimma Medical Center, Southwest Ethiopia.

Hailemichael T, Girma L, Fissiha P, Geteneh A, Kassa T BMC Infect Dis. 2023; 23(1):820.

PMID: 37993809 PMC: 10666304. DOI: 10.1186/s12879-023-08823-7.

Recent trends in the use of bacteriophages as replacement of antimicrobials against food-animal pathogens.

Zia S, Alkheraije K Front Vet Sci. 2023; 10:1162465.

PMID: 37303721 PMC: 10247982. DOI: 10.3389/fvets.2023.1162465.

Translating phage therapy into the clinic: Recent accomplishments but continuing challenges.

Petrovic Fabijan A, Iredell J, Danis-Wlodarczyk K, Kebriaei R, Abedon S PLoS Biol. 2023; 21(5):e3002119.

PMID: 37220114 PMC: 10204993. DOI: 10.1371/journal.pbio.3002119.

References

Bogovazova G, Voroshilova N, Bondarenko V, Gorbatkova G, Afanaseva E, Kazakova T . [Immunobiological properties and therapeutic effectiveness of preparations from Klebsiella bacteriophages]. Zh Mikrobiol Epidemiol Immunobiol. 1992; (3):30-3. View

Barrangou R, Yoon S, Breidt Jr F, Fleming H, Klaenhammer T . Characterization of six Leuconostoc fallax bacteriophages isolated from an industrial sauerkraut fermentation. Appl Environ Microbiol. 2002; 68(11):5452-8. PMC: 129880. DOI: 10.1128/AEM.68.11.5452-5458.2002. View

Matsuzaki S, Rashel M, Uchiyama J, Sakurai S, Ujihara T, Kuroda M . Bacteriophage therapy: a revitalized therapy against bacterial infectious diseases. J Infect Chemother. 2005; 11(5):211-9. DOI: 10.1007/s10156-005-0408-9. View

Pajunen M, Kiljunen S, Skurnik M . Bacteriophage phiYeO3-12, specific for Yersinia enterocolitica serotype O:3, is related to coliphages T3 and T7. J Bacteriol. 2000; 182(18):5114-20. PMC: 94659. DOI: 10.1128/JB.182.18.5114-5120.2000. View

Skurnik M, Pajunen M, Kiljunen S . Biotechnological challenges of phage therapy. Biotechnol Lett. 2007; 29(7):995-1003. DOI: 10.1007/s10529-007-9346-1. View

Chhibber S, Kaur S, Kumari S . Therapeutic potential of bacteriophage in treating Klebsiella pneumoniae B5055-mediated lobar pneumonia in mice. J Med Microbiol. 2008; 57(Pt 12):1508-1513. DOI: 10.1099/jmm.0.2008/002873-0. View

Carlton R . Phage therapy: past history and future prospects. Arch Immunol Ther Exp (Warsz). 1999; 47(5):267-74. View

Chang H, Chen C, Lin J, Shen G, Chang K, Tseng Y . Isolation and characterization of novel giant Stenotrophomonas maltophilia phage phiSMA5. Appl Environ Microbiol. 2005; 71(3):1387-93. PMC: 1065149. DOI: 10.1128/AEM.71.3.1387-1393.2005. View

Niemann H, Kwiatkowski B, WESTPHAL U, STIRM S . Klebsiella serotype 25 capsular polysaccharide: primary structure and depolymerization by a bacteriophage-borne glycanase. J Bacteriol. 1977; 130(1):366-74. PMC: 235214. DOI: 10.1128/jb.130.1.366-374.1977. View

10.

Wu L, Chang S, Yen M, Yang T, Tseng Y . Characterization of extended-host-range pseudo-T-even bacteriophage Kpp95 isolated on Klebsiella pneumoniae. Appl Environ Microbiol. 2007; 73(8):2532-40. PMC: 1855606. DOI: 10.1128/AEM.02113-06. View

11.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

12.

Mitra K, Ghosh A . Characterization of Vibrio cholerae O1 ElTor typing phage S5. Arch Virol. 2007; 152(10):1775-86. DOI: 10.1007/s00705-007-1021-2. View

13.

Sen A, Ghosh A . Physicochemical characterization of vibriophage N5. Virol J. 2005; 2:27. PMC: 1090621. DOI: 10.1186/1743-422X-2-27. View

14.

Yamamoto K, Alberts B, Benzinger R, Lawhorne L, Treiber G . Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology. 1970; 40(3):734-44. DOI: 10.1016/0042-6822(70)90218-7. View

15.

Watnick P, Kolter R . Biofilm, city of microbes. J Bacteriol. 2000; 182(10):2675-9. PMC: 101960. DOI: 10.1128/JB.182.10.2675-2679.2000. View

16.

Atterbury R, Connerton P, Dodd C, Rees C, Connerton I . Isolation and characterization of Campylobacter bacteriophages from retail poultry. Appl Environ Microbiol. 2003; 69(8):4511-8. PMC: 169066. DOI: 10.1128/AEM.69.8.4511-4518.2003. View

17.

Letkiewicz S, Miedzybrodzki R, Fortuna W, Weber-Dabrowska B, Gorski A . Eradication of Enterococcus faecalis by phage therapy in chronic bacterial prostatitis--case report. Folia Microbiol (Praha). 2009; 54(5):457-61. DOI: 10.1007/s12223-009-0064-z. View

18.

Shukla I, Tiwari R, Agrawal M . Prevalence of extended spectrum -lactamase producing Klebsiella pneumoniae in a tertiary care hospital. Indian J Med Microbiol. 2007; 22(2):87-91. View

19.

Kurzepa A, Dabrowska K, Switala-Jelen K, Gorski A . Molecular modification of T4 bacteriophage proteins and its potential application - review. Folia Microbiol (Praha). 2009; 54(1):5-15. DOI: 10.1007/s12223-009-0002-0. View

20.

Malik R, Chhibber S . Protection with bacteriophage KØ1 against fatal Klebsiella pneumoniae-induced burn wound infection in mice. J Microbiol Immunol Infect. 2009; 42(2):134-40. View