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Synthetic Polymer Coatings for Long-term Growth of Human Embryonic Stem Cells

Overview
Journal Nat Biotechnol
Specialty Biotechnology
Date 2010 Jun 1
PMID 20512122
Citations 136
Authors
Luis G Villa-Diaz
Himabindu Nandivada
Jun Ding
Naiara C Nogueira-de-Souza
Paul H Krebsbach
K Sue OShea
Joerg Lahann
Gary D Smith
Affiliations
Soon will be listed here.
Abstract

We report a fully defined synthetic polymer coating, poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), which sustains long-term human embryonic stem (hES) cell growth in several different culture media, including commercially available defined media. The development of a standardized, controllable and sustainable culture matrix for hES cells is an essential step in elucidating mechanisms that control hES cell behavior and in optimizing conditions for biomedical applications of hES cells.

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