Regulation of Arabidopsis Defense Responses Against Spodoptera Littoralis by CPK-mediated Calcium Signaling

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2010 May 28

PMID 20504319

Citations 58

Authors

Chidananda Nagamangala Kanchiswamy

Hirotaka Takahashi

Stefano Quadro

Massimo E Maffei

Simone Bossi

Cinzia Bertea

Simon Atsbaha Zebelo

Atsushi Muroi

Nobuaki Ishihama

Hirofumi Yoshioka

Wilhelm Boland

Junji Takabayashi

Yaeta Endo

Tatsuya Sawasaki

Gen-Ichiro Arimura

Affiliations

Soon will be listed here.

Abstract

Background: Plant Ca2+ signals are involved in a wide array of intracellular signaling pathways after pest invasion. Ca2+-binding sensory proteins such as Ca2+-dependent protein kinases (CPKs) have been predicted to mediate the signaling following Ca2+ influx after insect herbivory. However, until now this prediction was not testable.

Results: To investigate the roles CPKs play in a herbivore response-signaling pathway, we screened the characteristics of Arabidopsis CPK mutants damaged by a feeding generalist herbivore, Spodoptera littoralis. Following insect attack, the cpk3 and cpk13 mutants showed lower transcript levels of plant defensin gene PDF1.2 compared to wild-type plants. The CPK cascade was not directly linked to the herbivory-induced signaling pathways that were mediated by defense-related phytohormones such as jasmonic acid and ethylene. CPK3 was also suggested to be involved in a negative feedback regulation of the cytosolic Ca2+ levels after herbivory and wounding damage. In vitro kinase assays of CPK3 protein with a suite of substrates demonstrated that the protein phosphorylates transcription factors (including ERF1, HsfB2a and CZF1/ZFAR1) in the presence of Ca2+. CPK13 strongly phosphorylated only HsfB2a, irrespective of the presence of Ca2+. Furthermore, in vivo agroinfiltration assays showed that CPK3-or CPK13-derived phosphorylation of a heat shock factor (HsfB2a) promotes PDF1.2 transcriptional activation in the defense response.

Conclusions: These results reveal the involvement of two Arabidopsis CPKs (CPK3 and CPK13) in the herbivory-induced signaling network via HsfB2a-mediated regulation of the defense-related transcriptional machinery. This cascade is not involved in the phytohormone-related signaling pathways, but rather directly impacts transcription factors for defense responses.

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