Effects of Perfluorocarbons on Surfactant Exocytosis and Membrane Properties in Isolated Alveolar Type II Cells

Overview

Journal Respir Res

Specialty Pulmonary Medicine

Date 2010 May 13

PMID 20459693

Citations 3

Authors

Andreas Wemhoner

Irmgard Hackspiel

Nina Hobi

Andrea Ravasio

Thomas Haller

Mario Rudiger

Affiliations

Soon will be listed here.

Abstract

Background: Perfluorocarbons (PFC) are used to improve gas exchange in diseased lungs. PFC have been shown to affect various cell types. Thus, effects on alveolar type II (ATII) cells and surfactant metabolism can be expected, data, however, are controversial.

Objective: The study was performed to test two hypotheses: (I) the effects of PFC on surfactant exocytosis depend on their respective vapor pressures; (II) different pathways of surfactant exocytosis are affected differently by PFC.

Methods: Isolated ATII cells were exposed to two PFC with different vapor pressures and spontaneous surfactant exocytosis was measured. Furthermore, surfactant exocytosis was stimulated by either ATP, PMA or ionomycin. The effects of PFC on cell morphology, cellular viability, endocytosis, membrane permeability and fluidity were determined.

Results: The spontaneous exocytosis was reduced by PFC, however, the ATP and PMA stimulated exocytosis was slightly increased by PFC with high vapor pressure. In contrast, Ionomycin-induced exocytosis was decreased by PFC with low vapor pressure. Cellular uptake of FM 1-43 - a marker of membrane integrity - was increased. However, membrane fluidity, endocytosis and viability were not affected by PFC incubation.

Conclusions: We conclude that PFC effects can be explained by modest, unspecific interactions with the plasma membrane rather than by specific interactions with intracellular targets.

Citing Articles

Pulmonary surfactant preserves viability of alveolar type II cells exposed to polymyxin B in vitro.

Stichtenoth G, Herting E, Rudiger M, Wemhoner A PLoS One. 2013; 8(4):e62105.

PMID: 23620808 PMC: 3631157. DOI: 10.1371/journal.pone.0062105.

Persurf, a new method to improve surfactant delivery: a study in surfactant depleted rats.

Burkhardt W, Kraft S, Ochs M, Proquitte H, Mense L, Rudiger M PLoS One. 2012; 7(10):e47923.

PMID: 23082229 PMC: 3474734. DOI: 10.1371/journal.pone.0047923.

Perfluorochemical liquid-adenovirus suspensions enhance gene delivery to the distal lung.

Kazzaz J, Strayer M, Wu J, Malone D, Koo H, Shaffer T Pulm Med. 2011; 2011:918036.

PMID: 21876799 PMC: 3159382. DOI: 10.1155/2011/918036.

References

Burkhardt W, Koehne P, Wissel H, Graf S, Proquitte H, Wauer R . Intratracheal perfluorocarbons diminish LPS-induced increase in systemic TNF-alpha. Am J Physiol Lung Cell Mol Physiol. 2008; 294(6):L1043-8. DOI: 10.1152/ajplung.00125.2007. View

van Eeden S, Klut M, Leal M, Alexander J, Zonis Z, Skippen P . Partial liquid ventilation with perfluorocarbon in acute lung injury: light and transmission electron microscopy studies. Am J Respir Cell Mol Biol. 2000; 22(4):441-50. DOI: 10.1165/ajrcmb.22.4.3717. View

Leach C, Greenspan J, Rubenstein S, Shaffer T, Wolfson M, Jackson J . Partial liquid ventilation with perflubron in premature infants with severe respiratory distress syndrome. The LiquiVent Study Group. N Engl J Med. 1996; 335(11):761-7. DOI: 10.1056/NEJM199609123351101. View

Kubina M, Lanza F, Cazenave J, LAUSTRIAT G, Kuhry J . Parallel investigation of exocytosis kinetics and membrane fluidity changes in human platelets with the fluorescent probe, trimethylammonio-diphenylhexatriene. Biochim Biophys Acta. 1987; 901(1):138-46. DOI: 10.1016/0005-2736(87)90265-3. View

Rooney S . Regulation of surfactant secretion. Comp Biochem Physiol A Mol Integr Physiol. 2001; 129(1):233-43. DOI: 10.1016/s1095-6433(01)00320-8. View

Fernandez R, Sarma V, Younkin E, Hirschl R, Ward P, Younger J . Exposure to perflubron is associated with decreased Syk phosphorylation in human neutrophils. J Appl Physiol (1985). 2001; 91(5):1941-7. DOI: 10.1152/jappl.2001.91.5.1941. View

Chander A, Sen N, Spitzer A . Synexin and GTP increase surfactant secretion in permeabilized alveolar type II cells. Am J Physiol Lung Cell Mol Physiol. 2001; 280(5):L991-8. DOI: 10.1152/ajplung.2001.280.5.L991. View

Chintagari N, Jin N, Wang P, Narasaraju T, Chen J, Liu L . Effect of cholesterol depletion on exocytosis of alveolar type II cells. Am J Respir Cell Mol Biol. 2006; 34(6):677-87. PMC: 2644229. DOI: 10.1165/rcmb.2005-0418OC. View

Haller T, Dietl P, Pfaller K, Frick M, Mair N, Paulmichl M . Fusion pore expansion is a slow, discontinuous, and Ca2+-dependent process regulating secretion from alveolar type II cells. J Cell Biol. 2001; 155(2):279-89. PMC: 2198834. DOI: 10.1083/jcb.200102106. View

10.

Giocondi M, Mamdouh Z, Le Grimellec C . Benzyl alcohol differently affects fluid phase endocytosis and exocytosis in renal epithelial cells. Biochim Biophys Acta. 1995; 1234(2):197-202. DOI: 10.1016/0005-2736(94)00284-v. View

11.

Steinhorn D, Leach C, Fuhrman B, Holm B . Partial liquid ventilation enhances surfactant phospholipid production. Crit Care Med. 1996; 24(7):1252-6. DOI: 10.1097/00003246-199607000-00031. View

12.

Coster H . The physics of cell membranes. J Biol Phys. 2013; 29(4):363-99. PMC: 3456180. DOI: 10.1023/A:1027362704125. View

13.

Rudiger M, Wendt S, Kothe L, Burkhardt W, Wauer R, Ochs M . Alterations of alveolar type II cells and intraalveolar surfactant after bronchoalveolar lavage and perfluorocarbon ventilation. An electron microscopical and stereological study in the rat lung. Respir Res. 2007; 8:40. PMC: 1892019. DOI: 10.1186/1465-9921-8-40. View

14.

Haller T, Ortmayr J, Friedrich F, Volkl H, Dietl P . Dynamics of surfactant release in alveolar type II cells. Proc Natl Acad Sci U S A. 1998; 95(4):1579-84. PMC: 19102. DOI: 10.1073/pnas.95.4.1579. View

15.

Rotta A, Gunnarsson B, Hernan L, Fuhrman B, Steinhorn D . Partial liquid ventilation influences pulmonary histopathology in an animal model of acute lung injury. J Crit Care. 1999; 14(2):84-92. DOI: 10.1016/s0883-9441(99)90019-9. View

16.

Wemhoner A, Frick M, Dietl P, Jennings P, Haller T . A fluorescent microplate assay for exocytosis in alveolar type II cells. J Biomol Screen. 2006; 11(3):286-95. DOI: 10.1177/1087057105285284. View

17.

Modell J, Gollan F, Giammona S, Parker D . Effect of fluorocarbon liquid on surface tension properties of pulmonary surfactant. Chest. 1970; 57(3):263-5. DOI: 10.1378/chest.57.3.263. View

18.

Gladstone I, Ray A, Salafia C, Mercurio M, Jacobs H . Effect of artificial surfactant on pulmonary function in preterm and full-term lambs. J Appl Physiol (1985). 1990; 69(2):465-72. DOI: 10.1152/jappl.1990.69.2.465. View

19.

Sousa C, Nunes C, Lucio M, Ferreira H, Lima J, Tavares J . Effect of nonsteroidal anti-inflammatory drugs on the cellular membrane fluidity. J Pharm Sci. 2007; 97(8):3195-206. DOI: 10.1002/jps.21218. View

20.

Woods C, Neslund G, Kornbrust E, Flaim S . Perflubron attenuates neutrophil adhesion to activated endothelial cells in vitro. Am J Physiol Lung Cell Mol Physiol. 2000; 278(5):L1008-17. DOI: 10.1152/ajplung.2000.278.5.L1008. View