Tobacco Seeds Simultaneously Over-expressing Cu/Zn-superoxide Dismutase and Ascorbate Peroxidase Display Enhanced Seed Longevity and Germination Rates Under Stress Conditions

Overview

Journal J Exp Bot

Publisher Oxford University Press

Specialty Biology

Date 2010 Apr 29

PMID 20423937

Citations 27

Authors

Young Pyo Lee

Kwang-Hyun Baek

Haeng-Soon Lee

Sang-Soo Kwak

Jae-Woog Bang

Suk-Yoon Kwon

Affiliations

Soon will be listed here.

Abstract

Reactive oxygen species (ROS) are produced during seed desiccation, germination, and ageing, leading to cellular damage and seed deterioration and, therefore, decreased seed longevity. The effects of simultaneous over-expression of two antioxidant enzymes on seed longevity and seed germination under stressful conditions were investigated. Transgenic tobacco simultaneously over-expressing the Cu/Zn-superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) genes in plastids showed normal growth and seed development. Furthermore, the transgenic seeds displayed increased CuZnSOD and APX enzymatic activities during seed development and maintained antioxidant enzymatic activity after two years of dried storage at room temperature. The two-year stored non-transgenic seeds (aged NT seeds) had higher levels of ion leakage than the two-year stored transgenic seeds (aged CA seeds), indicating membrane damage caused by ROS was more severe in the aged NT seeds than the aged CA seeds. The aged CA seeds decreased germination rates as compared to newly harvested transgenic and non-transgenic seeds. The aged CA seeds, however, significantly increased germination rates under various abiotic stress conditions as compared to aged NT seeds. These data strongly suggest that simultaneous over-expression of the CuZnSOD and APX genes in plastids improves seed longevity and germination under various environmental stress conditions by attenuating the effects of oxidative stress produced by elongated storage conditions and harsh environmental stresses.

Citing Articles

Redox dynamics in seeds of spp: unraveling adaptation strategies of different seed categories.

Fuchs H, Staszak A, Vargas P, Sahrawy M, Serrato A, Dyderski M Front Plant Sci. 2024; 15:1430695.

PMID: 39114470 PMC: 11303208. DOI: 10.3389/fpls.2024.1430695.

and Enhance Seed Storability by Modulating Antioxidant Enzymes and Abscisic Acid in Rice.

Zheng X, Yuan Z, Yu Y, Yu S, He H Plants (Basel). 2024; 13(2).

PMID: 38276765 PMC: 10818270. DOI: 10.3390/plants13020310.

Noninvasive Methods to Detect Reactive Oxygen Species as a Proxy of Seed Quality.

Griffo A, Bosco N, Pagano A, Balestrazzi A, Macovei A Antioxidants (Basel). 2023; 12(3).

PMID: 36978875 PMC: 10045522. DOI: 10.3390/antiox12030626.

Transcription Factor DOF4.1 Regulates Seed Longevity in Arabidopsis Seed Permeability and Modulation of Seed Storage Protein Accumulation.

Ninoles R, Ruiz-Pastor C, Arjona-Mudarra P, Casan J, Renard J, Bueso E Front Plant Sci. 2022; 13:915184.

PMID: 35845633 PMC: 9284063. DOI: 10.3389/fpls.2022.915184.

GWAS and transcriptome analysis reveal MADS26 involved in seed germination ability in maize.

Ma L, Wang C, Hu Y, Dai W, Liang Z, Zou C Theor Appl Genet. 2022; 135(5):1717-1730.

PMID: 35247071 DOI: 10.1007/s00122-022-04065-4.

References

Becwar M, Stanwood P, Roos E . Dehydration effects on imbibitional leakage from desiccation-sensitive seeds. Plant Physiol. 1982; 69(5):1132-5. PMC: 426371. DOI: 10.1104/pp.69.5.1132. View

Beauchamp C, Fridovich I . Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem. 1971; 44(1):276-87. DOI: 10.1016/0003-2697(71)90370-8. View

Mittler R, Zilinskas B . Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought. Plant J. 1994; 5(3):397-405. DOI: 10.1111/j.1365-313x.1994.00397.x. View

Bowler C, Slooten L, VandenBranden S, De Rycke R, Botterman J, Sybesma C . Manganese superoxide dismutase can reduce cellular damage mediated by oxygen radicals in transgenic plants. EMBO J. 1991; 10(7):1723-32. PMC: 452843. DOI: 10.1002/j.1460-2075.1991.tb07696.x. View

Tang L, Kwon S, Kim S, Kim J, Choi J, Cho K . Enhanced tolerance of transgenic potato plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against oxidative stress and high temperature. Plant Cell Rep. 2006; 25(12):1380-6. DOI: 10.1007/s00299-006-0199-1. View

Payton P, Webb R, Kornyeyev D, Allen R, Holaday A . Protecting cotton photosynthesis during moderate chilling at high light intensity by increasing chloroplastic antioxidant enzyme activity. J Exp Bot. 2001; 52(365):2345-54. DOI: 10.1093/jexbot/52.365.2345. View

Bailly C, Audigier C, Ladonne F, Wagner M, Coste F, Corbineau F . Changes in oligosaccharide content and antioxidant enzyme activities in developing bean seeds as related to acquisition of drying tolerance and seed quality. J Exp Bot. 2001; 52(357):701-8. DOI: 10.1093/jexbot/52.357.701. View

Hendry G, Finch-Savage W, Thorpe P, Atherton N, Buckland S, Nilsson K . Free radical processes and loss of seed viability during desiccation in the recalcitrant species Quercus robur L. New Phytol. 2021; 122(2):273-279. DOI: 10.1111/j.1469-8137.1992.tb04231.x. View

Sunilkumar G, Mohr L, Lopata-Finch E, Emani C, Rathore K . Developmental and tissue-specific expression of CaMV 35S promoter in cotton as revealed by GFP. Plant Mol Biol. 2002; 50(3):463-74. DOI: 10.1023/a:1019832123444. View

10.

Ohlrogge J, Kernan T . Oxygen-dependent aging of seeds. Plant Physiol. 1982; 70(3):791-4. PMC: 1065772. DOI: 10.1104/pp.70.3.791. View

11.

Aono M, Saji H, Sakamoto A, Kondo N, Tanaka K . Paraquat tolerance of transgenic Nicotiana tabacum with enhanced activities of glutathione reductase and superoxide dismutase. Plant Cell Physiol. 1995; 36(8):1687-91. View

12.

Dat J, Vandenabeele S, Vranova E, Van Montagu M, Inze D, Van Breusegem F . Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci. 2000; 57(5):779-95. PMC: 11147059. DOI: 10.1007/s000180050041. View

13.

Allen R . Dissection of Oxidative Stress Tolerance Using Transgenic Plants. Plant Physiol. 1995; 107(4):1049-1054. PMC: 157235. DOI: 10.1104/pp.107.4.1049. View

14.

Kannan , Jain . Oxidative stress and apoptosis. Pathophysiology. 2000; 7(3):153-163. DOI: 10.1016/s0928-4680(00)00053-5. View

15.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

16.

Finkel T, Holbrook N . Oxidants, oxidative stress and the biology of ageing. Nature. 2000; 408(6809):239-47. DOI: 10.1038/35041687. View

17.

Smirnoff N . The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol. 2021; 125(1):27-58. DOI: 10.1111/j.1469-8137.1993.tb03863.x. View

18.

Fujimoto K, Neff W, Frankel E . The reaction of DNA with lipid oxidation products, metals and reducing agents. Biochim Biophys Acta. 1984; 795(1):100-7. DOI: 10.1016/0005-2760(84)90109-7. View

19.

Crowe J, Hoekstra F, Crowe L . Anhydrobiosis. Annu Rev Physiol. 1992; 54:579-99. DOI: 10.1146/annurev.ph.54.030192.003051. View

20.

Schopfer P, Plachy C, Frahry G . Release of reactive oxygen intermediates (superoxide radicals, hydrogen peroxide, and hydroxyl radicals) and peroxidase in germinating radish seeds controlled by light, gibberellin, and abscisic acid. Plant Physiol. 2001; 125(4):1591-602. PMC: 88817. DOI: 10.1104/pp.125.4.1591. View