Dietary Flavonoid Quercetin Stimulates Vasorelaxation in Aortic Vessels

Overview

Journal Free Radic Biol Med

Specialties Biochemistry
Biology
General Medicine

Date 2010 Apr 29

PMID 20423726

Citations 31

Authors

Nicholas K H Khoo

C Roger White

Lucas Pozzo-Miller

Fen Zhou

Chad Constance

Takafumi Inoue

Rakesh P Patel

Dale A Parks

Affiliations

Soon will be listed here.

Abstract

Considerable epidemiological evidence indicates that dietary consumption of moderate levels of polyphenols decreases both the incidence of cardiovascular disease and the mortality associated with myocardial infarction. Molecular mechanisms of this cardiovascular protection remain uncertain but can involve changes in rates of nitric oxide (NO) generation by endothelial nitric oxide synthase (eNOS). We examined the vascular responses to quercetin using a combination of biochemical and vessel function criteria. Quercetin treatment for 30min enhanced relaxation of rat aortic ring segments. Moreover, the addition of L-NAME (100muM) or charybdotoxin (ChTx) blocked quercetin-mediated vasorelaxation thus demonstrating the effect was partially dependent on NOS and endothelium-derived hyperpolarizing factor (EDHF). Additionally, bovine aortic endothelial cells (BAEC) treated with quercetin showed a rapid increase of intracellular Ca(2+) concentrations as well as a dose- and time-dependent stimulation of eNOS phosphorylation with a concomitant increase in NO production. These results demonstrate that quercetin-mediated stimulation of eNOS phosphorylation increases NO bioavailability in endothelial cells and can thus play a role in the vascular protective effects associated with improved endothelial cell function.

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