Zebrafish 10-formyltetrahydrofolate Dehydrogenase is Similar to Its Mammalian Isozymes for Its Structural and Catalytic Properties

Overview

Journal Protein Expr Purif

Specialty Molecular Biology

Date 2010 Apr 13

PMID 20381623

Citations 10

Authors

Wen-Ni Chang

Hung-Chang Lin

Tzu-Fun Fu

Affiliations

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Abstract

10-Formyltetrahydrofolate dehydrogenase from zebrafish has been cloned and expressed in both Escherichia coli and yeast. In addition, the N-terminal and C-terminal domains have also been cloned and expressed. Each expressed protein was purified to homogeneity and structural and kinetic properties determined. These studies show that the zebrafish enzyme is structurally and catalytically very similar to the enzymes from mammalian sources, suggesting that zebrafish can be used to study the in vivo function of 10-formyltetrahydrofolate dehydrogenase.

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