Trs85 Directs a Ypt1 GEF, TRAPPIII, to the Phagophore to Promote Autophagy

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2010 Apr 9

PMID 20375281

Citations 161

Authors

Molly A Lynch-Day

Deepali Bhandari

Shekar Menon

Ju Huang

Huaqing Cai

Clinton R Bartholomew

John H Brumell

Susan Ferro-Novick

Daniel J Klionsky

Affiliations

Soon will be listed here.

Abstract

Macroautophagy (hereafter autophagy) is a ubiquitous process in eukaryotic cells that is integrally involved in various aspects of cellular and organismal physiology. The morphological hallmark of autophagy is the formation of double-membrane cytosolic vesicles, autophagosomes, which sequester cytoplasmic cargo and deliver it to the lysosome or vacuole. Thus, autophagy involves dynamic membrane mobilization, yet the source of the lipid that forms the autophagosomes and the mechanism of membrane delivery are poorly characterized. The TRAPP complexes are multimeric guanine nucleotide exchange factors (GEFs) that activate the Rab GTPase Ypt1, which is required for secretion. Here we describe another form of this complex (TRAPPIII) that acts as an autophagy-specific GEF for Ypt1. The Trs85 subunit of the TRAPPIII complex directs this Ypt1 GEF to the phagophore assembly site (PAS) that is involved in autophagosome formation. Consistent with the observation that a Ypt1 GEF is directed to the PAS, we find that Ypt1 is essential for autophagy. This is an example of a Rab GEF that is specifically targeted for canonical autophagosome formation.

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