Induction of Cell Death in Neuroblastoma by Inhibition of Cathepsins B and L

Overview

Journal Cancer Lett

Specialty Oncology

Date 2010 Apr 6

PMID 20362389

Citations 10

Authors

Rita Colella

Guizhen Lu

Lisa Glazewski

Bruce Korant

Anjan Matlapudi

Matthew R England

Colin Craft

Christopher N Frantz

Robert W Mason

Affiliations

Soon will be listed here.

Abstract

A specific irreversible inhibitor of both cathepsins B and L, Fmoc-Tyr-Ala-CHN(2) (FYAD) induced apoptosis of neuroblastoma cells but not other tumor cells. Cysteine protease inhibitors that were not efficient inhibitors of both proteases did not cause death of any cell line tested. Apoptosis was preceded by accumulation of large electron dense vesicles and multivesicular bodies in the cytoplasm. Exposure of cells to the cathepsin D inhibitor, pepstatin, failed to rescue cells from FYAD-induced death. These results indicate that inhibition of cathepsins B and L may provide a unique mechanism for selectively inducing death of neuroblastoma with limited toxicity to normal cells and tissues.

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