Maternal Circulating CD34+VEGFR-2+ and CD133+VEGFR-2+ Progenitor Cells Increase During Normal Pregnancy but Are Reduced in Women with Preeclampsia

Overview

Journal Reprod Sci

Publisher Springer

Specialty Reproductive Medicine

Date 2010 Apr 3

PMID 20360595

Citations 29

Authors

Patrizia Luppi

Robert W Powers

Vivek Verma

Lia Edmunds

Daniel Plymire

Carl A Hubel

Affiliations

Soon will be listed here.

Abstract

Circulating endothelial progenitor cells (EPCs) may contribute to vascular endothelial cell homeostasis, and low levels of these cells are predictive of cardiovascular disease. We hypothesized that circulating EPCs increase in number during uncomplicated pregnancy but are reduced in women with preeclampsia. Peripheral blood was obtained from pregnant women and from nulligravidas in cross-sectional design. Cells expressing CD34 or CD133, in combination with vascular endothelial growth factor receptor-2 (VEGFR-2), were enumerated by flow cytometry. Both CD34(+)VEGFR-2(+) (doubly positive) and CD133(+)VEGFR-2( +) cells were significantly increased during the second and third trimesters of uncomplicated pregnancy compared to the first trimester. First trimester and nulligravida groups did not differ. Endothelial progenitor cells, quantified by flow cytometry or by circulating angiogenic cell (CAC) culture assay, were significantly reduced in women with preeclampsia compared to third trimester controls. Circulating EPCs appear to increase during normal pregnancy, and comparatively reduced numbers of these cells exist during preeclampsia.

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