Characterisation of Invasive Haemophilus Influenzae Isolates in Slovenia, 1993-2008

Overview

Journal Eur J Clin Microbiol Infect Dis

Publisher Springer

Specialties Infectious Diseases
Microbiology

Date 2010 Mar 23

PMID 20306323

Citations 8

Authors

T Kastrin

M Paragi

J Kolman

M Cizman

A Kraigher

M Gubina

Affiliations

Soon will be listed here.

Abstract

The objectives of our study were to describe the epidemiology of invasive Haemophilus influenzae disease from 1993 to 2008 in Slovenia, a country with routine H. influenzae serotype b (Hib) conjugate vaccination since the year 2000. A total of 292 isolates of H. influenzae, recovered from a normally sterile site, were collected in the study period. The isolates were serotyped by slide agglutination and antibiotic susceptibility was determined. One hundred and eight isolates received after the year 2000 were serotyped by slide agglutination and by polymerase chain reaction (PCR) capsule typing, and both methods were compared. After the introduction of the routine Hib vaccination, the incidence of H. influenzae disease in children under the age of 5 years has decreased by 87.6% and type b was replaced by non-typeable H. influenzae as the predominant serotype. The proportion of serotype b decreased from 85.3% in the pre-vaccination period to 13.0% in the vaccination period and the proportion of non-capsulated isolates increased from 12.0 to 85.2%. The study of genetic relatedness by pulsed-field gel electrophoresis (PFGE) demonstrated that the isolates of serotypes b and f were genetically homogeneous within the serotype. The results of our national surveillance showed that the vaccine has been very efficient in preventing Hib invasive disease in Slovenia. Nevertheless, we see a need for further monitoring of invasive H. influenzae infections at a national level.

Citing Articles

Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections.

Olie S, Andersen C, van de Beek D, Brouwer M Clin Microbiol Rev. 2024; 37(4):e0002124.

PMID: 39404267 PMC: 11629637. DOI: 10.1128/cmr.00021-24.

Fatal Meningitis and Sepsis Caused by Nontypeable .

Klibanov O, Kehr H, Jeter Z, Ekwonu T J Med Cases. 2022; 13(8):396-401.

PMID: 36128063 PMC: 9451564. DOI: 10.14740/jmc3974.

Epidemiology of Invasive Haemophilus influenzae Disease, Europe, 2007-2014.

Whittaker R, Economopoulou A, Gomes Dias J, Bancroft E, Ramliden M, Pastore Celentano L Emerg Infect Dis. 2017; 23(3):396-404.

PMID: 28220749 PMC: 5382729. DOI: 10.3201/eid2303.161552.

Association of influenza with severe pneumonia/empyema in the community, hospital, and healthcare-associated setting.

Seki M, Fuke R, Oikawa N, Hariu M, Watanabe Y Respir Med Case Rep. 2016; 19:1-4.

PMID: 27330964 PMC: 4908279. DOI: 10.1016/j.rmcr.2016.05.005.

Invasive Disease Caused by Nontypeable Haemophilus influenzae.

Langereis J, de Jonge M Emerg Infect Dis. 2015; 21(10):1711-8.

PMID: 26407156 PMC: 4593434. DOI: 10.3201/eid2110.150004.

References

Sarangi J, Cartwright K, Stuart J, Brookes S, Morris R, Slack M . Invasive Haemophilus influenzae disease in adults. Epidemiol Infect. 2000; 124(3):441-7. PMC: 2810930. DOI: 10.1017/s0950268899003611. View

Heath P, Booy R, Azzopardi H, Slack M, Fogarty J, Moloney A . Non-type b Haemophilus influenzae disease: clinical and epidemiologic characteristics in the Haemophilus influenzae type b vaccine era. Pediatr Infect Dis J. 2001; 20(3):300-5. DOI: 10.1097/00006454-200103000-00016. View

. Serotyping discrepancies in Haemophilus influenzae type b disease--United States, 1998-1999. MMWR Morb Mortal Wkly Rep. 2002; 51(32):706-7. View

Campos J, Roman F, Perez-Vazquez M, Aracil B, Oteo J, Cercenado E . Antibiotic resistance and clinical significance of Haemophilus influenzae type f. J Antimicrob Chemother. 2003; 52(6):961-6. DOI: 10.1093/jac/dkh004. View

Falla T, Crook D, Brophy L, Maskell D, Kroll J, Moxon E . PCR for capsular typing of Haemophilus influenzae. J Clin Microbiol. 1994; 32(10):2382-6. PMC: 264070. DOI: 10.1128/jcm.32.10.2382-2386.1994. View

Aracil B, Slack M, Perez-Vazquez M, Roman F, Ramsay M, Campos J . Molecular epidemiology of Haemophilus influenzae type b causing vaccine failures in the United Kingdom. J Clin Microbiol. 2006; 44(5):1645-9. PMC: 1479165. DOI: 10.1128/JCM.44.5.1645-1649.2006. View

van Ketel R, de Wever B, van Alphen L . Detection of Haemophilus influenzae in cerebrospinal fluids by polymerase chain reaction DNA amplification. J Med Microbiol. 1990; 33(4):271-6. DOI: 10.1099/00222615-33-4-271. View

Kim I, Ki C, Kim S, Oh W, Peck K, Song J . Diversity of ampicillin resistance genes and antimicrobial susceptibility patterns in Haemophilus influenzae strains isolated in Korea. Antimicrob Agents Chemother. 2006; 51(2):453-60. PMC: 1797734. DOI: 10.1128/AAC.00960-06. View

Peltola H . Worldwide Haemophilus influenzae type b disease at the beginning of the 21st century: global analysis of the disease burden 25 years after the use of the polysaccharide vaccine and a decade after the advent of conjugates. Clin Microbiol Rev. 2001; 13(2):302-17. PMC: 100154. DOI: 10.1128/CMR.13.2.302. View

10.

Cerquetti M, Cardines R, Giufre M, Mastrantonio P . Antimicrobial susceptibility of Haemophilus influenzae strains isolated from invasive disease in Italy. J Antimicrob Chemother. 2004; 54(6):1139-43. DOI: 10.1093/jac/dkh467. View

11.

Trotter C, Ramsay M, Slack M . Rising incidence of Haemophilus influenzae type b disease in England and Wales indicates a need for a second catch-up vaccination campaign. Commun Dis Public Health. 2003; 6(1):55-8. View

12.

Omikunle A, Takahashi S, Ogilvie C, Wang Y, Rodriguez C, St Geme 3rd J . Limited genetic diversity of recent invasive isolates of non-serotype b encapsulated Haemophilus influenzae. J Clin Microbiol. 2002; 40(4):1264-70. PMC: 140381. DOI: 10.1128/JCM.40.4.1264-1270.2002. View

13.

Tristram S, Jacobs M, Appelbaum P . Antimicrobial resistance in Haemophilus influenzae. Clin Microbiol Rev. 2007; 20(2):368-89. PMC: 1865592. DOI: 10.1128/CMR.00040-06. View

14.

Slack M, Azzopardi H, Hargreaves R, Ramsay M . Enhanced surveillance of invasive Haemophilus influenzae disease in England, 1990 to 1996: impact of conjugate vaccines. Pediatr Infect Dis J. 1998; 17(9 Suppl):S204-7. DOI: 10.1097/00006454-199809001-00026. View

15.

Garcia-Cobos S, Campos J, Lazaro E, Roman F, Cercenado E, Garcia-Rey C . Ampicillin-resistant non-beta-lactamase-producing Haemophilus influenzae in Spain: recent emergence of clonal isolates with increased resistance to cefotaxime and cefixime. Antimicrob Agents Chemother. 2007; 51(7):2564-73. PMC: 1913223. DOI: 10.1128/AAC.00354-07. View

16.

Rahman M, Hossain S, Baqui A, Shoma S, Rashid H, Nahar N . Haemophilus influenzae type-b and non-b-type invasive diseases in urban children (<5years) of Bangladesh: implications for therapy and vaccination. J Infect. 2008; 56(3):191-6. DOI: 10.1016/j.jinf.2007.12.008. View

17.

Saito M, Umeda A, Yoshida S . Subtyping of Haemophilus influenzae strains by pulsed-field gel electrophoresis. J Clin Microbiol. 1999; 37(7):2142-7. PMC: 85103. DOI: 10.1128/JCM.37.7.2142-2147.1999. View

18.

Jansen W, Verel A, Beitsma M, Verhoef J, Milatovic D . Longitudinal European surveillance study of antibiotic resistance of Haemophilus influenzae. J Antimicrob Chemother. 2006; 58(4):873-7. DOI: 10.1093/jac/dkl310. View

19.

Musser J, Kroll J, Granoff D, Moxon E, Brodeur B, Campos J . Global genetic structure and molecular epidemiology of encapsulated Haemophilus influenzae. Rev Infect Dis. 1990; 12(1):75-111. DOI: 10.1093/clinids/12.1.75. View

20.

Dworkin M, Park L, Borchardt S . The changing epidemiology of invasive Haemophilus influenzae disease, especially in persons > or = 65 years old. Clin Infect Dis. 2007; 44(6):810-6. DOI: 10.1086/511861. View