Size of AT(n) Insertions in Promoter Region Modulates Gmhsp17.6-L MRNA Transcript Levels

Overview

Journal J Biomed Biotechnol

Specialty Biology

Date 2010 Mar 20

PMID 20300590

Citations 5

Authors

Renata Fuganti

Maria de Fatima Pires da Silva Machado

Valeria Stefania Lopes

Joao Flavio Veloso Silva

Carlos Alberto Arrabal Arias

Silvana Regina Rockenbach Marin

Eliseu Binneck

Ricardo Vilela Abdelnoor

Francismar Correa Marcelino

Alexandre Lima Nepomuceno

Affiliations

Soon will be listed here.

Abstract

During earlier experiments, an SSR molecular marker (176 Soy HSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soybean. After being sequenced, results indicated that the SSR 176 Soy HSP marker was inserted in the promoter region of Gmhsp17.6-L gene. It was also detected in this region that resistant genotypes presented insertions between AT(31) and AT(33) in size and susceptible genotypes, AT(9). Gmhsp17.6-L gene coding region presented a perfect match in amino acid sequence in all soybean genotypes. A ribonuclease protection assay showed that Gmhsp17.6-L gene mRNA transcripts were present in all genotypes. A real-time relative quantification (qPCR) indicated in the resistant individuals higher mRNA transcripts levels, which presented in the sequencing more AT(n) insertions. These results suggest that the number of AT(n) insertions inside this promoter region could modulate up or down gene levels. Those findings can lead to the possibility of manipulating, between some limits, the mRNA transcripts levels using different sizes of AT(n) insertions.

Citing Articles

Genome-wide analysis of gene family in the Mediterranean olive ( subsp. ) provides insight into structural patterns, evolution and functional diversity.

Bettaieb I, Hamdi J, Bouktila D Physiol Mol Biol Plants. 2020; 26(11):2301-2318.

PMID: 33268931 PMC: 7688888. DOI: 10.1007/s12298-020-00888-x.

The soybean gene GmHsp22.4 is involved in the resistance response to Meloidogyne javanica in Arabidopsis thaliana.

Hishinuma-Silva S, Lopes-Caitar V, Nomura R, Sercero B, da Silva A, Carvalho M BMC Plant Biol. 2020; 20(1):535.

PMID: 33234121 PMC: 7687995. DOI: 10.1186/s12870-020-02736-2.

Heat Shock Proteins: Dynamic Biomolecules to Counter Plant Biotic and Abiotic Stresses.

Haq S, Khan A, Ali M, Khattak A, Gai W, Zhang H Int J Mol Sci. 2019; 20(21).

PMID: 31731530 PMC: 6862505. DOI: 10.3390/ijms20215321.

Genome-wide analysis and expression profiling under heat and drought treatments of HSP70 gene family in soybean (Glycine max L.).

Zhang L, Zhao H, Dong Q, Zhang Y, Wang Y, Li H Front Plant Sci. 2015; 6:773.

PMID: 26442082 PMC: 4585176. DOI: 10.3389/fpls.2015.00773.

Characterization and development of EST-SSR markers derived from transcriptome of yellow catfish.

Zhang J, Ma W, Song X, Lin Q, Gui J, Mei J Molecules. 2014; 19(10):16402-15.

PMID: 25314602 PMC: 6271634. DOI: 10.3390/molecules191016402.

References

Joshi C, Nguyen H . Differential display-mediated rapid identification of different members of a multigene family, HSP 16.9 in wheat. Plant Mol Biol. 1996; 31(3):575-84. DOI: 10.1007/BF00042230. View

Boston R, Viitanen P, Vierling E . Molecular chaperones and protein folding in plants. Plant Mol Biol. 1996; 32(1-2):191-222. DOI: 10.1007/BF00039383. View

Zhu J, Shi J, Bressan R, Hasegawa P . Expression of an Atriplex nummularia gene encoding a protein homologous to the bacterial molecular chaperone DnaJ. Plant Cell. 1993; 5(3):341-9. PMC: 160275. DOI: 10.1105/tpc.5.3.341. View

Bhatia P, Taylor W, Greenberg A, Wright J . Comparison of glyceraldehyde-3-phosphate dehydrogenase and 28S-ribosomal RNA gene expression as RNA loading controls for northern blot analysis of cell lines of varying malignant potential. Anal Biochem. 1994; 216(1):223-6. DOI: 10.1006/abio.1994.1028. View

Rieping M, Schoffl F . Synergistic effect of upstream sequences, CCAAT box elements, and HSE sequences for enhanced expression of chimaeric heat shock genes in transgenic tobacco. Mol Gen Genet. 1992; 231(2):226-32. DOI: 10.1007/BF00279795. View

Altschul S, Madden T, Schaffer A, Zhang J, Zhang Z, Miller W . Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997; 25(17):3389-402. PMC: 146917. DOI: 10.1093/nar/25.17.3389. View

Baniwal S, Bharti K, Chan K, Fauth M, Ganguli A, Kotak S . Heat stress response in plants: a complex game with chaperones and more than twenty heat stress transcription factors. J Biosci. 2004; 29(4):471-87. DOI: 10.1007/BF02712120. View

Ashley C, Pendleton C, Jennings W, Saxena A, Glover C . Isolation and sequencing of cDNA clones encoding Drosophila chromosomal protein D1. A repeating motif in proteins which recognize at DNA. J Biol Chem. 1989; 264(14):8394-401. View

Schoffl F, Schroder G, Kliem M, Rieping M . An SAR sequence containing 395 bp DNA fragment mediates enhanced, gene-dosage-correlated expression of a chimaeric heat shock gene in transgenic tobacco plants. Transgenic Res. 1993; 2(2):93-100. DOI: 10.1007/BF01969382. View

10.

Czarnecka E, Key J, GURLEY W . Regulatory domains of the Gmhsp17.5-E heat shock promoter of soybean. Mol Cell Biol. 1989; 9(8):3457-63. PMC: 362392. DOI: 10.1128/mcb.9.8.3457-3463.1989. View

11.

Ellis R, Hemmingsen S . Molecular chaperones: proteins essential for the biogenesis of some macromolecular structures. Trends Biochem Sci. 1989; 14(8):339-42. DOI: 10.1016/0968-0004(89)90168-0. View