A Simple Method for Concentration of Enteroviruses and Rotaviruses from Cell Culture Harvests Using Membrane Filters

Overview

Journal Intervirology

Specialty Microbiology

Date 1978 Jan 1

PMID 202572

Citations 11

Authors

S R Farrah

S M Goyal

C P Gerba

R H Conklin

C Wallis

J L Melnick

H L DuPont

Affiliations

Soon will be listed here.

Abstract

Organic compounds in cell culture harvests known as membrane-coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy-fiberglass filters. Subsequent elution with high pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100-fold.

Citing Articles

Simple method for the concentration of influenza virus from allantoic fluid on microporous filters.

Goyal S, Hanssen H, Gerba C Appl Environ Microbiol. 1980; 39(3):500-4.

PMID: 7387152 PMC: 291367. DOI: 10.1128/aem.39.3.500-504.1980.

Simple method for concentration of bacteria from large volumes of tap water.

Goyal S, Gerba C Appl Environ Microbiol. 1980; 40(5):912-6.

PMID: 7004354 PMC: 291689. DOI: 10.1128/aem.40.5.912-916.1980.

Round robin investigation of methods for the recovery of poliovirus from drinking water.

Melnick J, Safferman R, Rao V, Goyal S, Berg G, Dahling D Appl Environ Microbiol. 1984; 47(1):144-50.

PMID: 6320720 PMC: 239626. DOI: 10.1128/aem.47.1.144-150.1984.

Concentration of simian rotavirus SA-11 from tap water by membrane filtration and organic flocculation.

Guttman-Bass N, Armon R Appl Environ Microbiol. 1983; 45(3):850-5.

PMID: 6303220 PMC: 242382. DOI: 10.1128/aem.45.3.850-855.1983.

Development of a method for detection of human rotavirus in water and sewage.

Smith E, Gerba C Appl Environ Microbiol. 1982; 43(6):1440-50.

PMID: 6285825 PMC: 244252. DOI: 10.1128/aem.43.6.1440-1450.1982.