Identification of Diagnostic Urinary Biomarkers for Acute Kidney Injury

Overview

Journal J Investig Med

Publisher Sage Publications

Specialty General Medicine

Date 2010 Mar 13

PMID 20224435

Citations 7

Authors

Sanju A Varghese

Thomas B Powell

Michael G Janech

Milos N Budisavljevic

Romesh C Stanislaus

Jonas S Almeida

John M Arthur

Affiliations

Soon will be listed here.

Abstract

Acute kidney injury (AKI) is an important cause of death among hospitalized patients. The 2 most common causes of AKI are acute tubular necrosis (ATN) and prerenal azotemia (PRA). Appropriate diagnosis of the disease is important but often difficult. We analyzed urine proteins by 2-dimensional gel electrophoresis from 38 patients with AKI. Patients were randomly assigned to a training set, an internal test set, or an external validation set. Spot abundances were analyzed by artificial neural networks to identify biomarkers that differentiate between ATN and PRA. When the trained neural network algorithm was tested against the training data, it identified the diagnosis for 16 of 18 patients in the training set and all 10 patients in the internal test set. The accuracy was validated in the novel external set of patients where conditions of 9 of 10 patients were correctly diagnosed including 5 of 5 with ATN and 4 of 5 with PRA. Plasma retinol-binding protein was identified in 1 spot and a fragment of albumin and plasma retinol-binding protein in the other. These proteins are candidate markers for diagnostic assays of AKI.

Citing Articles

Acute Kidney Injury in Children: A Focus for the General Pediatrician.

Rivetti G, Gizzone P, Petrone D, Di Sessa A, Del Giudice E, Guarino S Children (Basel). 2024; 11(8).

PMID: 39201939 PMC: 11352805. DOI: 10.3390/children11081004.

The Clinical Significance of Urinary Retinol-Binding Protein 4: A Review.

Ratajczyk K, Konieczny A, Czekaj A, Piotrow P, Fiutowski M, Krakowska K Int J Environ Res Public Health. 2022; 19(16).

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Correlation Between Tenofovir Drug Levels and the Renal Biomarkers RBP-4 and ß2M in the ION-4 Study Cohort.

Chan A, Park L, Collins L, Cooper C, Saag M, Dieterich D Open Forum Infect Dis. 2019; 6(1):ofy273.

PMID: 30697570 PMC: 6344926. DOI: 10.1093/ofid/ofy273.

A comprehensive analysis and annotation of human normal urinary proteome.

Zhao M, Li M, Yang Y, Guo Z, Sun Y, Shao C Sci Rep. 2017; 7(1):3024.

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Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: predictors of patient prognosis?.

Gonzalez-Calero L, Martin-Lorenzo M, Ramos-Barron A, Ruiz-Criado J, Maroto A, Ortiz A Sci Rep. 2016; 6:19667.

PMID: 26792617 PMC: 4726181. DOI: 10.1038/srep19667.

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