A Comprehensive Analysis and Annotation of Human Normal Urinary Proteome

Overview

Journal Sci Rep

Specialty Science

Date 2017 Jun 10

PMID 28596590

Citations 72

Authors

Mindi Zhao

Menglin Li

Yehong Yang

Zhengguang Guo

Ying Sun

Chen Shao

Mingxi Li

Wei Sun

Youhe Gao

Affiliations

Soon will be listed here.

Abstract

Biomarkers are measurable changes associated with the disease. Urine can reflect the changes of the body while blood is under control of the homeostatic mechanisms; thus, urine is considered an important source for early and sensitive disease biomarker discovery. A comprehensive profile of the urinary proteome will provide a basic understanding of urinary proteins. In this paper, we present an in-depth analysis of the urinary proteome based on different separation strategies, including direct one dimensional liquid chromatography-tandem mass spectrometry (LC/MS/MS), two dimensional LC/MS/MS, and gel-eluted liquid fraction entrapment electrophoresis/liquid-phase isoelectric focusing followed by two dimensional LC/MS/MS. A total of 6085 proteins were identified in healthy urine, of which 2001 were not reported in previous studies and the concentrations of 2571 proteins were estimated (spanning a magnitude of 10) with an intensity-based absolute quantification algorithm. The urinary proteins were annotated by their tissue distribution. Detailed information can be accessed at the "Human Urine Proteome Database" (www.urimarker.com/urine).

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