Complete Nucleotide Sequence and Comparative Analysis of PPR9, a 41.7-kilobase Conjugative Staphylococcal Multiresistance Plasmid Conferring High-level Mupirocin Resistance

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2010 Mar 10

PMID 20211895

Citations 12

Authors

Eduardo Perez-Roth

Stephen M Kwong

Julia Alcoba-Florez

Neville Firth

Sebastian Mendez-Alvarez

Affiliations

Soon will be listed here.

Abstract

We have sequenced the conjugative plasmid pPR9, which carries the ileS2 gene, which had contributed to the dissemination of high-level mupirocin resistance at our institution. The plasmid backbone shows extensive genetic conservation with plasmids belonging to the pSK41/pGO1 family, but comparative analyses have revealed key differences that provide important insights into the evolution of these medically important plasmids and high-level mupirocin resistance in staphylococci and highlight the role of insertion sequence IS257 in these processes.

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References

LeBard R, Jensen S, Arnaiz I, Skurray R, Firth N . A multimer resolution system contributes to segregational stability of the prototypical staphylococcal conjugative multiresistance plasmid pSK41. FEMS Microbiol Lett. 2008; 284(1):58-67. DOI: 10.1111/j.1574-6968.2008.01190.x. View

Weaver K, Kwong S, Firth N, Francia M . The RepA_N replicons of Gram-positive bacteria: a family of broadly distributed but narrow host range plasmids. Plasmid. 2008; 61(2):94-109. PMC: 2652615. DOI: 10.1016/j.plasmid.2008.11.004. View

Kwong S, Skurray R, Firth N . Staphylococcus aureus multiresistance plasmid pSK41: analysis of the replication region, initiator protein binding and antisense RNA regulation. Mol Microbiol. 2004; 51(2):497-509. DOI: 10.1046/j.1365-2958.2003.03843.x. View

Simpson A, Skurray R, Firth N . An IS257-derived hybrid promoter directs transcription of a tetA(K) tetracycline resistance gene in the Staphylococcus aureus chromosomal mec region. J Bacteriol. 2000; 182(12):3345-52. PMC: 101884. DOI: 10.1128/JB.182.12.3345-3352.2000. View

Kwong S, Skurray R, Firth N . Replication control of staphylococcal multiresistance plasmid pSK41: an antisense RNA mediates dual-level regulation of Rep expression. J Bacteriol. 2006; 188(12):4404-12. PMC: 1482958. DOI: 10.1128/JB.00030-06. View

Diep B, Gill S, Chang R, Phan T, Chen J, Davidson M . Complete genome sequence of USA300, an epidemic clone of community-acquired meticillin-resistant Staphylococcus aureus. Lancet. 2006; 367(9512):731-9. DOI: 10.1016/S0140-6736(06)68231-7. View

Firth N, Skurray R . Mobile elements in the evolution and spread of multiple-drug resistance in staphylococci. Drug Resist Updat. 2006; 1(1):49-58. DOI: 10.1016/s1368-7646(98)80214-8. View

HODGSON J, Curnock S, DYKE K, Morris R, Sylvester D, Gross M . Molecular characterization of the gene encoding high-level mupirocin resistance in Staphylococcus aureus J2870. Antimicrob Agents Chemother. 1994; 38(5):1205-8. PMC: 188182. DOI: 10.1128/AAC.38.5.1205. View

Ewing B, Green P . Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res. 1998; 8(3):186-94. View

10.

Hurdle J, ONeill A, Mody L, Chopra I, Bradley S . In vivo transfer of high-level mupirocin resistance from Staphylococcus epidermidis to methicillin-resistant Staphylococcus aureus associated with failure of mupirocin prophylaxis. J Antimicrob Chemother. 2005; 56(6):1166-8. PMC: 3319406. DOI: 10.1093/jac/dki387. View

11.

Marchler-Bauer A, Anderson J, DeWeese-Scott C, Fedorova N, Geer L, He S . CDD: a curated Entrez database of conserved domain alignments. Nucleic Acids Res. 2003; 31(1):383-7. PMC: 165534. DOI: 10.1093/nar/gkg087. View

12.

Caryl J, ONeill A . Complete nucleotide sequence of pGO1, the prototype conjugative plasmid from the Staphylococci. Plasmid. 2009; 62(1):35-8. DOI: 10.1016/j.plasmid.2009.03.001. View

13.

Berg T, Firth N, Apisiridej S, Hettiaratchi A, Leelaporn A, Skurray R . Complete nucleotide sequence of pSK41: evolution of staphylococcal conjugative multiresistance plasmids. J Bacteriol. 1998; 180(17):4350-9. PMC: 107441. DOI: 10.1128/JB.180.17.4350-4359.1998. View

14.

Weigel L, Clewell D, Gill S, Clark N, McDougal L, Flannagan S . Genetic analysis of a high-level vancomycin-resistant isolate of Staphylococcus aureus. Science. 2003; 302(5650):1569-71. DOI: 10.1126/science.1090956. View

15.

Tettelin H, Radune D, Kasif S, Khouri H, Salzberg S . Optimized multiplex PCR: efficiently closing a whole-genome shotgun sequencing project. Genomics. 2000; 62(3):500-7. DOI: 10.1006/geno.1999.6048. View

16.

Grkovic S, Brown M, Hardie K, Firth N, Skurray R . Stable low-copy-number Staphylococcus aureus shuttle vectors. Microbiology (Reading). 2003; 149(Pt 3):785-794. DOI: 10.1099/mic.0.25951-0. View

17.

Simor A, Stuart T, Louie L, Watt C, Ofner-Agostini M, Gravel D . Mupirocin-resistant, methicillin-resistant Staphylococcus aureus strains in Canadian hospitals. Antimicrob Agents Chemother. 2007; 51(11):3880-6. PMC: 2151460. DOI: 10.1128/AAC.00846-07. View

18.

Cookson B . The emergence of mupirocin resistance: a challenge to infection control and antibiotic prescribing practice. J Antimicrob Chemother. 1998; 41(1):11-8. DOI: 10.1093/jac/41.1.11. View

19.

Udo E, Jacob L . Conjugative transfer of high-level mupirocin resistance and the mobilization of non-conjugative plasmids in Staphylococcus aureus. Microb Drug Resist. 1998; 4(3):185-93. DOI: 10.1089/mdr.1998.4.185. View

20.

Delcher A, Harmon D, Kasif S, White O, Salzberg S . Improved microbial gene identification with GLIMMER. Nucleic Acids Res. 1999; 27(23):4636-41. PMC: 148753. DOI: 10.1093/nar/27.23.4636. View