Nucleic Acid Detection Using MNAzymes

Overview

Journal Chem Biol

Publisher Elsevier

Specialties Biochemistry
Biology
Chemistry

Date 2010 Mar 2

PMID 20189100

Citations 17

Authors

Yulia V Gerasimova

Dmitry M Kolpashchikov

Affiliations

Soon will be listed here.

Abstract

Deoxyribozymes are promising biotechnological tools. In a recent JACS article, Mokany et al. reported on the design of multi-component deoxyribozyme (MNAzyme) sensors based on 10-23 and 8-17 DNA enzymes. The sensors can detect down to 5 pM of a specific nucleic acid. The versatility of MNAzyme platform allows the design of catalytic cascades for signal amplification. This work is a step forward to PCR-free molecular diagnostics.

Citing Articles

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Ateiah M, Gandalipov E, Rubel A, Rubel M, Kolpashchikov D Int J Mol Sci. 2023; 24(5).

PMID: 36901903 PMC: 10003685. DOI: 10.3390/ijms24054473.

Toward a Home Test for COVID-19 Diagnosis: DNA Machine for Amplification-Free SARS-CoV-2 Detection in Clinical Samples.

El-Deeb A, Zablotskaya S, Rubel M, Nour M, Kozlovskaya L, Shtro A ChemMedChem. 2022; 17(20):e202200382.

PMID: 36031581 PMC: 9538286. DOI: 10.1002/cmdc.202200382.

Role of biomaterials in the diagnosis, prevention, treatment, and study of corona virus disease 2019 (COVID-19).

Ertas Y, Mahmoodi M, Shahabipour F, Jahed V, Diltemiz S, Tutar R Emergent Mater. 2021; 4(1):35-55.

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Label-Free Pathogen Detection by a Deoxyribozyme Cascade with Visual Signal Readout.

Reed A, Connelly R, Williams A, Tran M, Shim B, Choe H Sens Actuators B Chem. 2019; 282:945-951.

PMID: 31462856 PMC: 6713451. DOI: 10.1016/j.snb.2018.11.147.

Species Typing of Nontuberculous Mycobacteria by Use of Deoxyribozyme Sensors.

Wood H, Sidders A, Brumsey L, Morozkin E, Gerasimova Y, Rohde K Clin Chem. 2018; 65(2):333-341.

PMID: 30523201 PMC: 6800115. DOI: 10.1373/clinchem.2018.295212.

References

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