Direct Culture-independent Strain Typing of Burkholderia Cepacia Complex in Sputum Samples from Patients with Cystic Fibrosis

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2010 Feb 26

PMID 20181898

Citations 6

Authors

Pavel Drevinek

Sarka Vosahlikova

Klara Dedeckova

Ondrej Cinek

Eshwar Mahenthiralingam

Affiliations

Soon will be listed here.

Abstract

We examined if multilocus sequence typing (MLST), a method for genotyping and species identification of Burkholderia cepacia complex bacteria, could be applied directly to cystic fibrosis sputum. The redesigned nested-PCR MLST format was successfully used to accurately identify strains in 23 sputum samples, of which 8 were culture negative.

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