Direct PCR Detection of Burkholderia Cepacia Complex and Identification of Its Genomovars by Using Sputum As Source of DNA

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2002 Aug 31

PMID 12202599

Citations 7

Authors

Pavel Drevinek

Hana Hrbackova

Ondrej Cinek

Jana Bartosova

Otakar Nyc

Alexandr Nemec

Petr Pohunek

Affiliations

Soon will be listed here.

Abstract

We developed a nested PCR assay that detects the recA gene of the Burkholderia cepacia complex in sputum. The product of the first PCR round is also used to identify the genomovar of the pathogen. The protocol achieves high sensitivity and specificity with simple interpretation of genomovar status.

Citing Articles

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Direct culture-independent Strain typing of Burkholderia cepacia complex in sputum samples from patients with cystic fibrosis.

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Diversity of the parB and repA genes of the Burkholderia cepacia complex and their utility for rapid identification of Burkholderia cenocepacia.

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