Detoxified Endotoxin Vaccine (J5dLPS/OMP) Protects Mice Against Lethal Respiratory Challenge with Francisella Tularensis SchuS4

Overview

Journal Vaccine

Specialty Allergy & Immunology

Date 2010 Feb 23

PMID 20170768

Citations 5

Authors

Stephen H Gregory

Wilbur H Chen

Stephanie Mott

John E Palardy

Nicholas A Parejo

Sara Heninger

Christine A Anderson

Andrew W Artenstein

Steven M Opal

Alan S Cross

Affiliations

Soon will be listed here.

Abstract

Francisella tularensis is a category A select agent. J5dLPS/OMP is a novel vaccine construct consisting of detoxified, O-polysaccharide side chain-deficient, lipopolysaccharide non-covalently complexed with the outer membrane protein of N. meningitidis group B. Immunization elicits high-titer polyclonal antibodies specific for the highly-conserved epitopes expressed within the glycolipid core that constitutes gram-negative bacteria (e.g., F. tularensis). Mice immunized intranasally with J5dLPS/OMP exhibited protective immunity to intratracheal challenge with the live vaccine strain, as well as the highly-virulent SchuS4 strain, of F. tularensis. The efficacy of J5dLPS/OMP vaccine suggests its potential utility in immunizing the general population against several different gram-negative select agents concurrently.

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