Ubiquitin Chain Elongation Enzyme Ufd2 Regulates a Subset of Doa10 Substrates

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2010 Feb 18

PMID 20159987

Citations 19

Authors

Chang Liu

Dewald van Dyk

Ping Xu

Vitnary Choe

Haihui Pan

Junmin Peng

Brenda Andrews

Hai Rao

Affiliations

Soon will be listed here.

Abstract

Ufd2 is the founding member of E4 enzymes that are specifically involved in ubiquitin chain elongation but whose roles in proteolysis remain scarce. Here, using a genome-wide screen, we identified one cellular target of yeast Ufd2 as the membrane protein Pex29. The ubiquitin chains assembled on Pex29 in vivo by Ufd2 mainly contain Lys-48 linkages. We found that the ubiquitin-protein E3 ligase for overexpressed Pex29 is Doa10, which is known to be involved in protein quality control. Interestingly, not all Doa10 substrates are regulated by Ufd2, suggesting that E4 involvement is not specific to a particular E3, but may depend on the spatial arrangement of the E3-substrate interaction. Cells lacking UFD2 elicit an unfolded protein response, expanding the physiological function of Ufd2. Our results lead to novel insights into the biological role of Ufd2 and further underscore the significance of Ufd2 in proteolysis.

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