Time-resolved Fluorescence Spectroscopy As a Diagnostic Technique of Oral Carcinoma: Validation in the Hamster Buccal Pouch Model

Overview

Journal Arch Otolaryngol Head Neck Surg

Publisher American Medical Association

Specialty Otorhinolaryngology

Date 2010 Feb 17

PMID 20157056

Citations 15

Authors

D Gregory Farwell

Jeremy D Meier

Jesung Park

Yang Sun

Heather Coffman

Brian Poirier

Jennifer Phipps

Steve Tinling

Danny J Enepekides

Laura Marcu

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the benefit of using time-resolved, laser-induced fluorescence spectroscopy for diagnosing malignant and premalignant lesions of the oral cavity.

Design: The carcinogen 7,12-dimethylbenz[a]anthracene (DMBA) was applied to 1 cheek pouch of 19 hamsters. The contralateral pouch and the cheek pouches of 3 hamsters without DMBA exposure served as controls.

Setting: University of California, Davis.

Participants: Twenty-two golden/Syrian hamsters.

Intervention: A nitrogen pulse laser was used to induce tissue autofluorescence between the wavelengths of 360 and 650 nm.

Main Outcome Measures: Spectral intensities and time-domain measurements were obtained and compared with the histopathologic findings at each corresponding site.

Results: Spectral intensities and lifetime values at 3 spectral bands (SBs; SB1 = 380 +/- 10 nm; SB2 = 460 +/- 10 nm, and SB3 = 635 +/- 10 nm) allowed for discrimination among healthy epithelium, dysplasia, carcinoma in situ, and invasive carcinoma. The lifetime values at SB2 were the most important when distinguishing the lesions using only time-resolved parameters. An algorithm combining spectral fluorescence parameters derived from both spectral and time-domain parameters (peak intensities, average fluorescence lifetimes, and the Laguerre coefficient [zero-order]) for healthy epithelium, dysplasia, carcinoma in situ, and invasive carcinoma provided the best diagnostic discrimination, with 100%, 100%, 69.2%, and 76.5% sensitivity and 100%, 92.2%, 97.1%, and 96.2% specificity, respectively.

Conclusions: The addition of time-resolved fluorescence-derived parameters significantly improves the capability of fluorescence spectroscopy-based diagnostics in the hamster buccal pouch. This technique provides a potential noninvasive diagnostic instrument for head and neck cancer.

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References

Mallia R, Thomas S, Mathews A, Kumar R, Sebastian P, Madhavan J . Laser-induced autofluorescence spectral ratio reference standard for early discrimination of oral cancer. Cancer. 2008; 112(7):1503-12. DOI: 10.1002/cncr.23324. View

Panjehpour M, Overholt B, Vo-Dinh T, Haggitt R, Edwards D, Buckley 3rd F . Endoscopic fluorescence detection of high-grade dysplasia in Barrett's esophagus. Gastroenterology. 1996; 111(1):93-101. DOI: 10.1053/gast.1996.v111.pm8698231. View

Marcu L, Fang Q, Jo J, Papaioannou T, Dorafshar A, Reil T . In vivo detection of macrophages in a rabbit atherosclerotic model by time-resolved laser-induced fluorescence spectroscopy. Atherosclerosis. 2005; 181(2):295-303. PMC: 2672099. DOI: 10.1016/j.atherosclerosis.2005.02.010. View

Gimenez-Conti I . The hamster cheek pouch carcinogenesis model. Acta Odontol Latinoam. 1993; 7(1):3-12. View

Schwarz R, Gao W, Daye D, Williams M, Richards-Kortum R, Gillenwater A . Autofluorescence and diffuse reflectance spectroscopy of oral epithelial tissue using a depth-sensitive fiber-optic probe. Appl Opt. 2008; 47(6):825-34. PMC: 2773166. DOI: 10.1364/ao.47.000825. View

Fang Q, Papaioannou T, Jo J, Vaitha R, Shastry K, Marcu L . Time-domain laser-induced fluorescence spectroscopy apparatus for clinical diagnostics. Rev Sci Instrum. 2022; 75(1):151-162. PMC: 8920500. DOI: 10.1063/1.1634354. View

Coghlan L, Utzinger U, Richards-Kortum R, Brookner C, Zuluaga A, Gimenez-Conti I . Fluorescence spectroscopy of epithelial tissue throughout the dysplasia-carcinoma sequence in an animal model: spectroscopic changes precede morphologic changes. Lasers Surg Med. 2001; 29(1):1-10. DOI: 10.1002/lsm.1078. View

Chen C, Chiang H, Chow S, Wang C, Lee Y, Tsai J . Autofluorescence in normal and malignant human oral tissues and in DMBA-induced hamster buccal pouch carcinogenesis. J Oral Pathol Med. 1998; 27(10):470-4. DOI: 10.1111/j.1600-0714.1998.tb01914.x. View

Schomacker K, Frisoli J, Compton C, Flotte T, Richter J, Nishioka N . Ultraviolet laser-induced fluorescence of colonic tissue: basic biology and diagnostic potential. Lasers Surg Med. 1992; 12(1):63-78. DOI: 10.1002/lsm.1900120111. View

10.

Butte P, Pikul B, Hever A, Yong W, Black K, Marcu L . Diagnosis of meningioma by time-resolved fluorescence spectroscopy. J Biomed Opt. 2006; 10(6):064026. PMC: 2981341. DOI: 10.1117/1.2141624. View

11.

Chen H, Chiang C, You C, Hsiao T, Wang C . Time-resolved autofluorescence spectroscopy for classifying normal and premalignant oral tissues. Lasers Surg Med. 2005; 37(1):37-45. DOI: 10.1002/lsm.20192. View

12.

Heintzelman D, Utzinger U, Fuchs H, Zuluaga A, Gossage K, Gillenwater A . Optimal excitation wavelengths for in vivo detection of oral neoplasia using fluorescence spectroscopy. Photochem Photobiol. 2000; 72(1):103-13. DOI: 10.1562/0031-8655(2000)072<0103:OEWFIV>2.0.CO;2. View

13.

Poh C, Zhang L, Anderson D, Durham J, Williams P, Priddy R . Fluorescence visualization detection of field alterations in tumor margins of oral cancer patients. Clin Cancer Res. 2006; 12(22):6716-22. DOI: 10.1158/1078-0432.CCR-06-1317. View

14.

de Veld D, Witjes M, Sterenborg H, Roodenburg J . The status of in vivo autofluorescence spectroscopy and imaging for oral oncology. Oral Oncol. 2005; 41(2):117-31. DOI: 10.1016/j.oraloncology.2004.07.007. View

15.

SALLEY J . Experimental carcinogenesis in the cheek pouch of the Syrian hamster. J Dent Res. 1954; 33(2):253-62. DOI: 10.1177/00220345540330021201. View

16.

Gillenwater A, Jacob R, Ganeshappa R, Kemp B, El-Naggar A, Palmer J . Noninvasive diagnosis of oral neoplasia based on fluorescence spectroscopy and native tissue autofluorescence. Arch Otolaryngol Head Neck Surg. 1998; 124(11):1251-8. DOI: 10.1001/archotol.124.11.1251. View

17.

Wang C, Chen C, Chiang C, Young S, Chow S, Chiang H . A probability-based multivariate statistical algorithm for autofluorescence spectroscopic identification of oral carcinogenesis. Photochem Photobiol. 1999; 69(4):471-7. View

18.

Ramanujam N, Mitchell M, Mahadevan A, Warren S, Thomsen S, Silva E . In vivo diagnosis of cervical intraepithelial neoplasia using 337-nm-excited laser-induced fluorescence. Proc Natl Acad Sci U S A. 1994; 91(21):10193-7. PMC: 44984. DOI: 10.1073/pnas.91.21.10193. View

19.

Wang C, Tsai T, Chen H, Chang S, Chen C, Chiang C . Autofluorescence spectroscopy for in vivo diagnosis of DMBA-induced hamster buccal pouch pre-cancers and cancers. J Oral Pathol Med. 2003; 32(1):18-24. DOI: 10.1034/j.1600-0714.2003.00049.x. View

20.

Meier J, Enepekides D, Poirier B, Bradley C, Albala J, Farwell D . Treatment with 1-alpha,25-dihydroxyvitamin D3 (vitamin D3) to inhibit carcinogenesis in the hamster buccal pouch model. Arch Otolaryngol Head Neck Surg. 2007; 133(11):1149-52. DOI: 10.1001/archotol.133.11.1149. View