Organelle-specific, Rapid Induction of Molecular Activities and Membrane Tethering

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2010 Feb 16

PMID 20154678

Citations 82

Authors

Toru Komatsu

Igor Kukelyansky

J Michael McCaffery

Tasuku Ueno

Lidenys C Varela

Takanari Inoue

Affiliations

Soon will be listed here.

Abstract

Using new chemically inducible dimerization probes, we generated a system to rapidly target proteins to individual intracellular organelles. Using this system, we activated Ras GTPase at distinct intracellular locations and induced tethering of membranes from two organelles, endoplasmic reticulum and mitochondria. Innovative techniques to rapidly perturb molecular activities and organelle-organelle communications at precise locations and timing will provide powerful strategies to dissect spatiotemporally complex biological processes.

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