Characterization of Harpy/Rca1/emi1 Mutants: Patterning in the Absence of Cell Division

Overview

Journal Dev Dyn

Publisher Wiley

Specialty Anatomy & Histology

Date 2010 Feb 11

PMID 20146251

Citations 9

Authors

Bruce B Riley

Elly M Sweet

Rebecca Heck

Adrienne Evans

Karen N McFarland

Rachel M Warga

Donald A Kane

Affiliations

Soon will be listed here.

Abstract

We have characterized mutations in the early arrest gene, harpy (hrp), and show that they introduce premature stops in the coding region of early mitotic inhibitor1 (Rca1/emi1). In harpy mutants, cells stop dividing during early gastrulation. Lineage analysis confirms that there is little change in cell number after approximately cycle-14. Gross patterning occurs relatively normally, and many organ primordia are produced on time but with smaller numbers of cells. Despite the lack of cell division, some organ systems continue to increase in cell number, suggesting recruitment from surrounding areas. Analysis of bromodeoxyuridine incorporation shows that endoreduplication continues in many cells well past the first day of development, but cells cease endoreduplication once they begin to differentiate and express cell-type markers. Despite relatively normal gross patterning, harpy mutants show several defects in morphogenesis, cell migration and differentiation resulting directly or indirectly from the arrest of cell division.

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