Mesodermal Fgf10b Cooperates with Other Fibroblast Growth Factors During Induction of Otic and Epibranchial Placodes in Zebrafish

Overview

Journal Dev Dyn

Publisher Wiley

Specialty Anatomy & Histology

Date 2014 Mar 29

PMID 24677486

Citations 8

Authors

Kirstin Maulding

Mahesh S Padanad

Jennifer Dong

Bruce B Riley

Affiliations

Soon will be listed here.

Abstract

Background: Vertebrate otic and epibranchial placodes develop in close proximity in response to localized fibroblast growth factor (Fgf) signaling. Although less is known about epibranchial induction, the process of otic induction in highly conserved, with important roles for Fgf3 and Fgf8 reported in all species examined. Fgf10 is also critical for otic induction in mouse, but the only zebrafish ortholog examined to date, fgf10a, is not expressed early enough to play such a role. A second zebrafish ortholog, fgf10b, has not been previously examined.

Results: We find that zebrafish fgf10b is expressed at tailbud stage in paraxial cephalic mesoderm beneath prospective epibranchial tissue, lateral to the developing otic placode. Knockdown of fgf10b does not affect initial otic induction but impairs subsequent accumulation of otic cells. Formation of epibranchial placodes and ganglia are also moderately impaired. Combinatorial disruption of fgf10b and fgf3 exacerbates the deficiency of otic cells and eliminates epibranchial induction entirely. Disruption of fgf10b and fgf24 also strongly reduces, but does not eliminate, epibranchial induction.

Conclusions: fgf10b participates in a late phase of otic induction and, in combination with fgf3, is especially critical for epibranchial induction.

Citing Articles

Fgf, Hh, and pax2a differentially regulate expression of pax5 and pou3f3b in vestibular and auditory maculae in the zebrafish otic vesicle.

Tan A, Christensen S, Baker A, Riley B Dev Dyn. 2023; 252(10):1269-1279.

PMID: 37171017 PMC: 10712688. DOI: 10.1002/dvdy.599.

Development and evolution of the vestibular apparatuses of the inner ear.

Mackowetzky K, Yoon K, Mackowetzky E, Waskiewicz A J Anat. 2021; 239(4):801-828.

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The Warburg Effect and lactate signaling augment Fgf-MAPK to promote sensory-neural development in the otic vesicle.

Kantarci H, Gou Y, Riley B Elife. 2020; 9.

PMID: 32338604 PMC: 7253172. DOI: 10.7554/eLife.56301.

sox2 and sox3 cooperate to regulate otic/epibranchial placode induction in zebrafish.

Gou Y, Guo J, Maulding K, Riley B Dev Biol. 2018; 435(1):84-95.

PMID: 29355522 PMC: 5818308. DOI: 10.1016/j.ydbio.2018.01.011.

Dlx3b/4b is required for early-born but not later-forming sensory hair cells during zebrafish inner ear development.

Schwarzer S, Spiess S, Brand M, Hans S Biol Open. 2017; 6(9):1270-1278.

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