Long-term Characteristics of Hazelnut Allergy in an Adjuvant-free Mouse Model

Overview

Journal Int Arch Allergy Immunol

Specialty Allergy & Immunology

Date 2010 Feb 11

PMID 20145410

Citations 12

Authors

Babu Gonipeta

Sitaram Parvataneni

Pranati Paruchuri

Venu Gangur

Affiliations

Soon will be listed here.

Abstract

Background: Clinically it is recognized that tree nut allergies such as hazelnut allergy are not usually outgrown. Specific mechanisms underlying the persistence of such food allergies are incompletely understood. Here we studied the natural history and the long-term immune and clinical characteristics of hazelnut allergy in an adjuvant-free mouse model.

Methods: BALB/c mice were sensitized to hazelnut protein using a transdermal sensitization protocol that does not use adjuvant. After establishing sensitization, exposure to hazelnut was withdrawn for 3, 5 or 8 months. The fate of circulating IgE antibodies was monitored. Subsequently, mice were given booster exposures and examined for memory IgE antibody and spleen cell IL-4 responses. Clinical characteristics and hypothermia responses upon oral allergen challenge were studied.

Results: Upon allergen withdrawal, circulating hazelnut-specific IgE antibody levels began to drop. Nevertheless, IgE responses once established remained at significantly high levels for up to 8 months (the last time point studied) despite withdrawal of allergen exposure. Memory IgE responses to booster exposures were robust after 3, 5 or 8 months of allergen withdrawal. Furthermore, significant clinical reactivity to oral hazelnut challenge, and hypothermia responses were demonstrable at each of these time points. Long-lasting spleen cell memory IL-4 responses to hazelnut were detectable in these mice explaining the mechanism of sustenance of IgE responses and clinical sensitization.

Conclusions: Hazelnut allergy once established persists for long periods, despite withdrawal of allergen exposure, due to long-lasting, memory IgE and IL-4 responses.

Citing Articles

An Adjuvant-Free Mouse Model Using Skin Sensitization Without Tape-Stripping Followed by Oral Elicitation of Anaphylaxis: A Novel Pre-Clinical Tool for Testing Intrinsic Wheat Allergenicity.

Gao H, Jorgensen R, Raghunath R, Ng P, Gangur V Front Allergy. 2022; 3:926576.

PMID: 36238931 PMC: 9552944. DOI: 10.3389/falgy.2022.926576.

A Mouse-Based Method to Monitor Wheat Allergens in Novel Wheat Lines and Varieties Created by Crossbreeding: Proof-of-Concept Using Durum and Wheats.

Jorgensen R, Raghunath R, Gao H, Olson E, Ng P, Gangur V Int J Mol Sci. 2022; 23(12).

PMID: 35742949 PMC: 9224339. DOI: 10.3390/ijms23126505.

Mechanisms of Wheat Allergenicity in Mice: Comparison of Adjuvant-Free vs. Alum-Adjuvant Models.

Jin Y, Gao H, Jorgensen R, Salloum J, Jian D, Ng P Int J Mol Sci. 2020; 21(9).

PMID: 32369940 PMC: 7247356. DOI: 10.3390/ijms21093205.

Advances in Molecular Mechanisms of Wheat Allergenicity in Animal Models: A Comprehensive Review.

Jin Y, Acharya H, Acharya D, Jorgensen R, Gao H, Secord J Molecules. 2019; 24(6).

PMID: 30909404 PMC: 6471126. DOI: 10.3390/molecules24061142.

Murine models for mucosal tolerance in allergy.

Smole U, Schabussova I, Pickl W, Wiedermann U Semin Immunol. 2017; 30:12-27.

PMID: 28807539 PMC: 7100013. DOI: 10.1016/j.smim.2017.07.007.

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