Identification and Characterization of a Non-satellite Cell Muscle Resident Progenitor During Postnatal Development

Overview

Journal Nat Cell Biol

Specialty Cell Biology

Date 2010 Feb 2

PMID 20118923

Citations 220

Authors

Kathryn J Mitchell

Alice Pannerec

Bruno Cadot

Ara Parlakian

Vanessa Besson

Edgar R Gomes

Giovanna Marazzi

David A Sassoon

Affiliations

Soon will be listed here.

Abstract

Satellite cells are resident myogenic progenitors in postnatal skeletal muscle involved in muscle postnatal growth and adult regenerative capacity. Here, we identify and describe a population of muscle-resident stem cells, which are located in the interstitium, that express the cell stress mediator PW1 but do not express other markers of muscle stem cells such as Pax7. PW1(+)/Pax7(-) interstitial cells (PICs) are myogenic in vitro and efficiently contribute to skeletal muscle regeneration in vivo as well as generating satellite cells and PICs. Whereas Pax7 mutant satellite cells show robust myogenic potential, Pax7 mutant PICs are unable to participate in myogenesis and accumulate during postnatal growth. Furthermore, we found that PICs are not derived from a satellite cell lineage. Taken together, our findings uncover a new and anatomically identifiable population of muscle progenitors and define a key role for Pax7 in a non-satellite cell population during postnatal muscle growth.

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References

Lepper C, Conway S, Fan C . Adult satellite cells and embryonic muscle progenitors have distinct genetic requirements. Nature. 2009; 460(7255):627-31. PMC: 2767162. DOI: 10.1038/nature08209. View

Montarras D, Morgan J, Collins C, Relaix F, Zaffran S, Cumano A . Direct isolation of satellite cells for skeletal muscle regeneration. Science. 2005; 309(5743):2064-7. DOI: 10.1126/science.1114758. View

Seale P, Ishibashi J, Scime A, Rudnicki M . Pax7 is necessary and sufficient for the myogenic specification of CD45+:Sca1+ stem cells from injured muscle. PLoS Biol. 2004; 2(5):E130. PMC: 406392. DOI: 10.1371/journal.pbio.0020130. View

Mauro A . Satellite cell of skeletal muscle fibers. J Biophys Biochem Cytol. 1961; 9:493-5. PMC: 2225012. DOI: 10.1083/jcb.9.2.493. View

Relaix F, Wei X, Wu X, Sassoon D . Peg3/Pw1 is an imprinted gene involved in the TNF-NFkappaB signal transduction pathway. Nat Genet. 1998; 18(3):287-91. DOI: 10.1038/ng0398-287. View

Charge S, Rudnicki M . Cellular and molecular regulation of muscle regeneration. Physiol Rev. 2004; 84(1):209-38. DOI: 10.1152/physrev.00019.2003. View

Bischoff R . Proliferation of muscle satellite cells on intact myofibers in culture. Dev Biol. 1986; 115(1):129-39. DOI: 10.1016/0012-1606(86)90234-4. View

Asakura A, Seale P, Girgis-Gabardo A, Rudnicki M . Myogenic specification of side population cells in skeletal muscle. J Cell Biol. 2002; 159(1):123-34. PMC: 2173497. DOI: 10.1083/jcb.200202092. View

Sacco A, Doyonnas R, Kraft P, Vitorovic S, Blau H . Self-renewal and expansion of single transplanted muscle stem cells. Nature. 2008; 456(7221):502-6. PMC: 2919355. DOI: 10.1038/nature07384. View

10.

Hughes D, Ontell M . Morphometric analysis of the developing, murine aneural soleus muscle. Dev Dyn. 1992; 193(2):175-84. DOI: 10.1002/aja.1001930209. View

11.

Ontell M, Kozeka K . The organogenesis of murine striated muscle: a cytoarchitectural study. Am J Anat. 1984; 171(2):133-48. DOI: 10.1002/aja.1001710202. View

12.

Tamaki T, Akatsuka A, Yoshimura S, Roy R, Edgerton V . New fiber formation in the interstitial spaces of rat skeletal muscle during postnatal growth. J Histochem Cytochem. 2002; 50(8):1097-111. DOI: 10.1177/002215540205000812. View

13.

Tamaki T, Akatsuka A, Ando K, Nakamura Y, Matsuzawa H, Hotta T . Identification of myogenic-endothelial progenitor cells in the interstitial spaces of skeletal muscle. J Cell Biol. 2002; 157(4):571-7. PMC: 2173851. DOI: 10.1083/jcb.200112106. View

14.

Moura-Neto V, Kryszke M, Li Z, Vicart P, Lilienbaum A, Paulin D . A 28-bp negative element with multiple factor-binding activity controls expression of the vimentin-encoding gene. Gene. 1996; 168(2):261-6. DOI: 10.1016/0378-1119(95)00789-x. View

15.

Johnson M, Wu X, Aithmitti N, Morrison R . Peg3/Pw1 is a mediator between p53 and Bax in DNA damage-induced neuronal death. J Biol Chem. 2002; 277(25):23000-7. DOI: 10.1074/jbc.M201907200. View

16.

Olguin H, Olwin B . Pax-7 up-regulation inhibits myogenesis and cell cycle progression in satellite cells: a potential mechanism for self-renewal. Dev Biol. 2004; 275(2):375-88. PMC: 3322464. DOI: 10.1016/j.ydbio.2004.08.015. View

17.

Bischoff R . Regeneration of single skeletal muscle fibers in vitro. Anat Rec. 1975; 182(2):215-35. DOI: 10.1002/ar.1091820207. View

18.

Schwarzkopf M, Coletti D, Sassoon D, Marazzi G . Muscle cachexia is regulated by a p53-PW1/Peg3-dependent pathway. Genes Dev. 2006; 20(24):3440-52. PMC: 1698450. DOI: 10.1101/gad.412606. View

19.

Montarras D, Lindon C, Pinset C, Domeyne P . Cultured myf5 null and myoD null muscle precursor cells display distinct growth defects. Biol Cell. 2001; 92(8-9):565-72. DOI: 10.1016/s0248-4900(00)01110-2. View

20.

Polesskaya A, Seale P, Rudnicki M . Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration. Cell. 2003; 113(7):841-52. DOI: 10.1016/s0092-8674(03)00437-9. View