Urate Crystals Stimulate Production of Tumor Necrosis Factor Alpha from Human Blood Monocytes and Synovial Cells. Cytokine MRNA and Protein Kinetics, and Cellular Distribution

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1991 Apr 1

PMID 2010550

Citations 72

Authors

F S Di Giovine

S E Malawista

E Thornton

G W Duff

Affiliations

Soon will be listed here.

Abstract

Crystals of monosodium urate (MSU) provide a dose-dependent stimulus for the production by human blood monocytes of tumor necrosis factor (TNF), a cytokine with proinflammatory properties; TNF activity was inhibited selectively by monoclonal antibody to TNF alpha. Biologically active cell-associated TNF activity peaked at 3 h and was exceeded at 6 h by extracellular activity, which peaked at 12-18 h. Comparable kinetics were observed with immunoreactive TNF alpha. TNF alpha mRNA accumulation in monocytes stimulated with MSU crystals appeared as a single peak at 2-4 h, kinetics compatible with rapid production of a short half-life transcript. In contrast, crystals of calcium pyrophosphate or of hydroxyapatite did not stimulate significant production of TNF or of message. Fresh tophaceous material from a patient with gout contained significant levels of TNF alpha and cells cultured from the tophus produced TNF alpha in vitro. In rheumatoid synovial cells, spontaneous release of TNF alpha was increased by in vitro exposure to MSU crystals. Taken together with earlier work, these results support an expanded view of gouty inflammation in which the crystal-stimulated production of cytokines provides a crucial link between crystal deposition and many of the clinical and pathological facts of both acute and chronic gouty arthritis.

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References

Hanson D, Murphy P, Windle B . Failure of rabbit neutrophils to secrete endogenous pyrogen when stimulated with staphylococci. J Exp Med. 1980; 151(6):1360-71. PMC: 2185872. DOI: 10.1084/jem.151.6.1360. View

Decker T, Lohmann-Matthes M, Gifford G . Cell-associated tumor necrosis factor (TNF) as a killing mechanism of activated cytotoxic macrophages. J Immunol. 1987; 138(3):957-62. View

Feramisco J, Smart J, Burridge K, Helfman D, Thomas G . Co-existence of vinculin and a vinculin-like protein of higher molecular weight in smooth muscle. J Biol Chem. 1982; 257(18):11024-31. View

Duff G, Atkins E . The detection of endotoxin by in vitro production of endogenous pyrogen: comparison with limulus amebocyte lysate gelation. J Immunol Methods. 1982; 52(3):323-31. DOI: 10.1016/0022-1759(82)90004-7. View

Flick D, Gifford G . Comparison of in vitro cell cytotoxic assays for tumor necrosis factor. J Immunol Methods. 1984; 68(1-2):167-75. DOI: 10.1016/0022-1759(84)90147-9. View

Dinarello C . Interleukin-1. Rev Infect Dis. 1984; 6(1):51-95. DOI: 10.1093/clinids/6.1.51. View

Feinberg A, Vogelstein B . "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984; 137(1):266-7. DOI: 10.1016/0003-2697(84)90381-6. View

Duff G, Atkins E, Malawista S . The fever of gout: urate crystals activate endogenous pyrogen production from human and rabbit mononuclear phagocytes. Trans Assoc Am Physicians. 1983; 96:234-45. View

Pennica D, Nedwin G, Hayflick J, Seeburg P, Derynck R, Palladino M . Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin. Nature. 1984; 312(5996):724-9. DOI: 10.1038/312724a0. View

10.

Kaczmarek L, Calabretta B, Baserga R . Expression of cell-cycle-dependent genes in phytohemagglutinin-stimulated human lymphocytes. Proc Natl Acad Sci U S A. 1985; 82(16):5375-9. PMC: 390571. DOI: 10.1073/pnas.82.16.5375. View

11.

Torti F, DIECKMANN B, Beutler B, Cerami A, Ringold G . A macrophage factor inhibits adipocyte gene expression: an in vitro model of cachexia. Science. 1985; 229(4716):867-9. DOI: 10.1126/science.3839597. View

12.

Malawista S, Duff G, Atkins E, Cheung H, McCarty D . Crystal-induced endogenous pyrogen production. A further look at gouty inflammation. Arthritis Rheum. 1985; 28(9):1039-46. DOI: 10.1002/art.1780280911. View

13.

Marmenout A, Fransen L, Tavernier J, Van der Heyden J, Tizard R, Kawashima E . Molecular cloning and expression of human tumor necrosis factor and comparison with mouse tumor necrosis factor. Eur J Biochem. 1985; 152(3):515-22. DOI: 10.1111/j.1432-1033.1985.tb09226.x. View

14.

Dayer J, Beutler B, Cerami A . Cachectin/tumor necrosis factor stimulates collagenase and prostaglandin E2 production by human synovial cells and dermal fibroblasts. J Exp Med. 1985; 162(6):2163-8. PMC: 2187983. DOI: 10.1084/jem.162.6.2163. View

15.

Collins T, Lapierre L, Fiers W, Strominger J, Pober J . Recombinant human tumor necrosis factor increases mRNA levels and surface expression of HLA-A,B antigens in vascular endothelial cells and dermal fibroblasts in vitro. Proc Natl Acad Sci U S A. 1986; 83(2):446-50. PMC: 322876. DOI: 10.1073/pnas.83.2.446. View

16.

Bertolini D, Nedwin G, Bringman T, Smith D, Mundy G . Stimulation of bone resorption and inhibition of bone formation in vitro by human tumour necrosis factors. Nature. 1986; 319(6053):516-8. DOI: 10.1038/319516a0. View

17.

Beutler B, Cerami A . Cachectin and tumour necrosis factor as two sides of the same biological coin. Nature. 1986; 320(6063):584-8. DOI: 10.1038/320584a0. View

18.

Beutler B, Krochin N, Milsark I, Luedke C, Cerami A . Control of cachectin (tumor necrosis factor) synthesis: mechanisms of endotoxin resistance. Science. 1986; 232(4753):977-80. DOI: 10.1126/science.3754653. View

19.

Dinarello C, Cannon J, Wolff S, Bernheim H, Beutler B, Cerami A . Tumor necrosis factor (cachectin) is an endogenous pyrogen and induces production of interleukin 1. J Exp Med. 1986; 163(6):1433-50. PMC: 2188124. DOI: 10.1084/jem.163.6.1433. View

20.

Saklatvala J . Tumour necrosis factor alpha stimulates resorption and inhibits synthesis of proteoglycan in cartilage. Nature. 1986; 322(6079):547-9. PMC: 7095107. DOI: 10.1038/322547a0. View