Human Beta-defensin 3 Has Immunosuppressive Activity in Vitro and in Vivo

Overview

Journal Eur J Immunol

Specialty Allergy & Immunology

Date 2010 Jan 28

PMID 20104491

Citations 80

Authors

Fiona Semple

Sheila Webb

Hsin-Ni Li

Hetal B Patel

Mauro Perretti

Ian J Jackson

Mohini Gray

Donald J Davidson

Julia R Dorin

Affiliations

Soon will be listed here.

Abstract

Beta-defensins are antimicrobial peptides with an essential role in the innate immune response. In addition beta-defensins can also chemoattract cells involved in adaptive immunity. Until now, based on evidence from dendritic cell stimulation, human beta defensin-3 (hBD3) was considered pro-inflammatory. We present evidence here that hBD3 lacks pro-inflammatory activity in human and mouse primary Mphi. In addition, in the presence of LPS, hBD3 and the murine orthologue Defb14 (but not hBD2), effectively inhibit TNF-alpha and IL-6 accumulation implying an anti-inflammatory function. hBD3 also inhibits CD40/IFN-gamma stimulation of Mphi and in vivo, hBD3 significantly reduces the LPS-induced TNF-alpha level in serum. Recent work has revealed that hBD3 binds melanocortin receptors but we provide evidence that these are not involved in hBD3 immunomodulatory activity. This implies a dual role for hBD3 in antimicrobial activity and resolution of inflammation.

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