D-Xylose As a Repressor or Inducer of Xylanase Expression in Hypocrea Jecorina (Trichoderma Reesei)

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2010 Jan 26

PMID 20097821

Citations 30

Authors

Astrid R Mach-Aigner

Marion E Pucher

Robert L Mach

Affiliations

Soon will be listed here.

Abstract

For Hypocrea jecorina (anamorph Trichoderma reesei), a filamentous fungus used for hydrolase production in different industries, it has been a long-term practice to use d-xylose as an inducing substance. We demonstrate in this study that the degree of xylanase-encoding gene induction strictly depends on the concentration of d-xylose, which was found to be optimal from 0.5 to 1 mM for 3 h of cultivation. At higher concentrations of d-xylose, a reduced level of xylanase gene expression was observed. In the present study, we also provide evidence that the d-xylose concentration-dependent induction is antagonized by carbon catabolite repressor 1. This repressor mediates its influence on d-xylose indirectly, by reducing the expression of xylanase regulator 1, the main activator of most hydrolase-encoding genes. Additionally, a direct influence of the repressor on xylanase 1 expression in the presence of d-xylose was found. Furthermore, we show that d-xylose reductase 1 is needed to metabolize d-xylose to achieve full induction of xylanase expression. Finally, a strain which expresses xylanase regulator 1 at a constant level was used to partially overcome the negative influence exerted by carbon catabolite repressor 1 on d-xylose.

Citing Articles

In Vitro Characterization of a Nuclear Receptor-like Domain of the Xylanase Regulator 1 from .

Mello-de-Sousa T, Gorsche R, Jovanovic B, Mach R, Mach-Aigner A J Fungi (Basel). 2022; 8(12).

PMID: 36547587 PMC: 9784857. DOI: 10.3390/jof8121254.

Factors regulating cellulolytic gene expression in filamentous fungi: an overview.

Mattam A, Chaudhari Y, Velankar H Microb Cell Fact. 2022; 21(1):44.

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Elimination of the Sugar Transporter GAT1 Increased Xylanase I Production in .

Xu W, Fang Y, Ding M, Ren Y, Meng X, Chen G Front Microbiol. 2022; 13:810066.

PMID: 35154055 PMC: 8825865. DOI: 10.3389/fmicb.2022.810066.

An overview on current molecular tools for heterologous gene expression in Trichoderma.

Tomico-Cuenca I, Mach R, Mach-Aigner A, Derntl C Fungal Biol Biotechnol. 2021; 8(1):11.

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Cellulase Production of () by Continuously Fed Cultivation Using Sucrose as Primary Carbon Source.

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