Two Cellobiohydrolase-encoding Genes from Aspergillus Niger Require D-xylose and the Xylanolytic Transcriptional Activator XlnR for Their Expression

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1999 Oct 3

PMID 10508057

Citations 70

Authors

M M Gielkens

E Dekkers

J Visser

L H de Graaff

Affiliations

Soon will be listed here.

Abstract

Two cellobiohydrolase-encoding genes, cbhA and cbhB, have been isolated from the filamentous fungus Aspergillus niger. The deduced amino acid sequence shows that CbhB has a modular structure consisting of a fungus-type cellulose-binding domain (CBD) and a catalytic domain separated by a Pro/Ser/Thr-rich linker peptide. CbhA consists only of a catalytic domain and lacks a CBD and linker peptide. Both proteins are homologous to fungal cellobiohydrolases in family 7 of the glycosyl hydrolases. Northern blot analysis showed that the transcription of the cbhA and cbhB genes is induced by D-xylose but not by sophorose and, in addition, requires the xylanolytic transcriptional activator XlnR.

Citing Articles

Effects of hap2 deletion on mnp/vp transcription in Pleurotus ostreatus grown on lignocellulosic substrates.

Kayama K, Nakazawa T, Yamaguchi I, Kawauchi M, Sakamoto M, Honda Y Appl Microbiol Biotechnol. 2024; 108(1):513.

PMID: 39535609 PMC: 11561020. DOI: 10.1007/s00253-024-13352-7.

Independent metabolism of oligosaccharides is the keystone of synchronous utilization of cellulose and hemicellulose in .

Liu J, Chen M, Gu S, Fan R, Zhao Z, Sun W PNAS Nexus. 2024; 3(2):pgae053.

PMID: 38380057 PMC: 10877092. DOI: 10.1093/pnasnexus/pgae053.

Comparative Genomics and Transcriptomics Analyses Reveal Divergent Plant Biomass-Degrading Strategies in Fungi.

Li J, Wiebenga A, Lipzen A, Ng V, Tejomurthula S, Zhang Y J Fungi (Basel). 2023; 9(8).

PMID: 37623631 PMC: 10455118. DOI: 10.3390/jof9080860.

In Vitro Characterization of a Nuclear Receptor-like Domain of the Xylanase Regulator 1 from .

Mello-de-Sousa T, Gorsche R, Jovanovic B, Mach R, Mach-Aigner A J Fungi (Basel). 2022; 8(12).

PMID: 36547587 PMC: 9784857. DOI: 10.3390/jof8121254.

MtTRC-1, a Novel Transcription Factor, Regulates Cellulase Production via Directly Modulating the Genes Expression of the and in .

Li N, Liu Y, Liu D, Liu D, Zhang C, Lin L Appl Environ Microbiol. 2022; 88(19):e0126322.

PMID: 36165620 PMC: 9552611. DOI: 10.1128/aem.01263-22.

References

van Peij N, Gielkens M, de Vries R, Visser J, de Graaff L . The transcriptional activator XlnR regulates both xylanolytic and endoglucanase gene expression in Aspergillus niger. Appl Environ Microbiol. 1998; 64(10):3615-9. PMC: 106473. DOI: 10.1128/AEM.64.10.3615-3619.1998. View

Carpita N, Gibeaut D . Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth. Plant J. 1993; 3(1):1-30. DOI: 10.1111/j.1365-313x.1993.tb00007.x. View

de Vries R, Visser J, de Graaff L . CreA modulates the XlnR-induced expression on xylose of Aspergillus niger genes involved in xylan degradation. Res Microbiol. 1999; 150(4):281-5. DOI: 10.1016/s0923-2508(99)80053-9. View

Harmsen J, Kester H, Visser J . Structure of the Aspergillus niger pelA gene and its expression in Aspergillus niger and Aspergillus nidulans. Curr Genet. 1991; 20(4):293-9. DOI: 10.1007/BF00318518. View

Saloheimo M, Lehtovaara P, Penttila M, Teeri T, Stahlberg J, Johansson G . EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme. Gene. 1988; 63(1):11-22. DOI: 10.1016/0378-1119(88)90541-0. View

Azevedo M, Felipe M, Astolfi-Filho S, Radford A . Cloning, sequencing and homologies of the cbh-1 (exoglucanase) gene of Humicola grisea var. thermoidea. J Gen Microbiol. 1990; 136(12):2569-76. DOI: 10.1099/00221287-136-12-2569. View

Zeilinger S, Mach R, Kubicek C . Two adjacent protein binding motifs in the cbh2 (cellobiohydrolase II-encoding) promoter of the fungus Hypocrea jecorina (Trichoderma reesei) cooperate in the induction by cellulose. J Biol Chem. 1998; 273(51):34463-71. DOI: 10.1074/jbc.273.51.34463. View

Srisodsuk M, Lehtio J, Linder M, Margolles-Clark E, Reinikainen T, Teeri T . Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain: action on bacterial microcrystalline cellulose. J Biotechnol. 1997; 57(1-3):49-57. DOI: 10.1016/s0168-1656(97)00088-6. View

Gielkens M, Visser J, de Graaff L . Arabinoxylan degradation by fungi: characterization of the arabinoxylan-arabinofuranohydrolase encoding genes from Aspergillus niger and Aspergillus tubingensis. Curr Genet. 1997; 31(1):22-9. DOI: 10.1007/s002940050172. View

10.

Hrmova M, Petrakova E, Biely P . Induction of cellulose- and xylan-degrading enzyme systems in Aspergillus terreus by homo- and heterodisaccharides composed of glucose and xylose. J Gen Microbiol. 1991; 137(3):541-7. DOI: 10.1099/00221287-137-3-541. View

11.

Kitamoto N, Yoshino S, Ito M, Kimura T, Ohmiya K, Tsukagoshi N . Repression of the expression of genes encoding xylanolytic enzymes in Aspergillus oryzae by introduction of multiple copies of the xynF1 promoter. Appl Microbiol Biotechnol. 1998; 50(5):558-63. DOI: 10.1007/s002530051334. View

12.

de Graaff L, van den Broeck H, van Ooijen A, Visser J . Regulation of the xylanase-encoding xlnA gene of Aspergillus tubigensis. Mol Microbiol. 1994; 12(3):479-90. DOI: 10.1111/j.1365-2958.1994.tb01036.x. View

13.

de Graaff L, Van den Broeck H, Visser J . Isolation and characterization of the Aspergillus niger pyruvate kinase gene. Curr Genet. 1992; 22(1):21-7. DOI: 10.1007/BF00351737. View

14.

von Heijne G . A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 1986; 14(11):4683-90. PMC: 311474. DOI: 10.1093/nar/14.11.4683. View

15.

Ilmen M, SALOHEIMO A, Onnela M, Penttila M . Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei. Appl Environ Microbiol. 1997; 63(4):1298-306. PMC: 168424. DOI: 10.1128/aem.63.4.1298-1306.1997. View

16.

Melchers W, Verweij P, Van Den Hurk P, van Belkum A, De Pauw B, Hoogkamp-Korstanje J . General primer-mediated PCR for detection of Aspergillus species. J Clin Microbiol. 1994; 32(7):1710-7. PMC: 263770. DOI: 10.1128/jcm.32.7.1710-1717.1994. View

17.

Covert S, Vanden Wymelenberg A, Cullen D . Structure, organization, and transcription of a cellobiohydrolase gene cluster from Phanerochaete chrysosporium. Appl Environ Microbiol. 1992; 58(7):2168-75. PMC: 195751. DOI: 10.1128/aem.58.7.2168-2175.1992. View

18.

Kubicek C, Messner R, Gruber F, Mach R . The Trichoderma cellulase regulatory puzzle: from the interior life of a secretory fungus. Enzyme Microb Technol. 1993; 15(2):90-99. DOI: 10.1016/0141-0229(93)90030-6. View

19.

Henrissat B, Bairoch A . Updating the sequence-based classification of glycosyl hydrolases. Biochem J. 1996; 316 ( Pt 2):695-6. PMC: 1217404. DOI: 10.1042/bj3160695. View

20.

Beguin P . Molecular biology of cellulose degradation. Annu Rev Microbiol. 1990; 44:219-48. DOI: 10.1146/annurev.mi.44.100190.001251. View