Proteomic Analysis of Heparin-binding Proteins from Human Seminal Plasma: a Step Towards Identification of Molecular Markers of Male Fertility

Overview

Journal J Biosci

Specialties Biochemistry
Biology

Date 2010 Jan 23

PMID 20093743

Citations 19

Authors

Vijay Kumar

Md Imtaiyaz Hassan

Anil Kumar Tomar

Tara Kashav

Jaya Nautiyal

Sarman Singh

Tej P Singh

Savita Yadav

Affiliations

Soon will be listed here.

Abstract

Glycosaminoglycans, especially heparin, are involved in various cell processes such as apoptosis, cell cycle control, platelet activation, capacitation, acrosome reaction and sperm decondensation. Heparin-binding proteins (HBPs) are essential constituents of human seminal fluid, which bind to sperm lipids containing the phosphorylcholine group and mediate the fertilization process. We utilized a proteomic set-up consisting of affinity chromatography, isoelectric focusing (IEF) coupled with matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI TOF/MS) for protein analysis of human HBPs. We resolved 70 different spots on two-dimensional (2-D) gel and subsequently identifi ed these proteins. Forty different types of proteins were identified. Functional analysis revealed that 38% of the proteins belonged to the enzyme category, 20% were involved in RNA processing and transcription, 18% in structure and transport function, and 16% in cell recognition and signal transduction. We also identified 8% of proteins with unknown functions, although their expression in seminal fluid has been documented. Proteins of seminal fluid that bind heparin may be directly involved in sperm capacitation and acrosome reaction (AR), which are the two critical steps for fertilization. This information on HBPs would be useful for identifying potential biomarkers of fertility in the near future.

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